中国兽医科学2017，Vol.47Issue(3)：286-294,9.DOI:10.16656/j.issn.1673-4696.2017.03.003
鸭源致病性大肠杆菌的分离鉴定及系统发育分析
Isolation, identification and phylogenetic analysis of avian pathogenic Escherichia coli from duck
摘要
Abstract
In order to determine the pathogenic bacteria in a duck-farm from Xuzhou City of Jiangsu Province,a strain,named as JSE4,was isolated from the liver of a dead duck,identified as Escherichia coli based on its colony morphology,microscopic characteristics,biochemical test and 16S rRNA sequence.Rapid detection of avian pathogenic E.coli,virulence assay,virulence genes,drug sensitive test and phylogenetic analysis showed that the strain was avian pathogenic E.coli,and it could cause 16.7％ death of the experimental group of ducks.The isolate contained 12 of 14 virulence genes.The result of resistance test to 30 drugs showed that the strain was highly sensitive to streptomycin,macrodantin,imipenem,furazolidone,ceftazidime and aztreonam.16S rRNA sequence analysis showed that the strain had a close genetic distance with strains from Belgium water,Switzerland poultry meat and China duck,the strain had 99.7％homology to isolates from Belgium water and Switzerland poultry meat.The above results indicate that a avian pathogenic E.coli strain with multiple drug resistance (MDR) is isolated from the liver of a dead duck in a duck-farm from Xuzhou City of Jiangsu Province.关键词
禽致病性大肠杆菌/鸭/分离鉴定/药敏试验/系统发育学分析Key words
avian pathogenic E.coli/duck/isolation and identification/medicine sensitive test/phylogenetic analysis分类
农业科技引用本文复制引用
孙畅,赵丽丽,徐丽晶,牛银杰,尹海畅,陆涛峰,张圆圆,弥春霞,陈洪岩..鸭源致病性大肠杆菌的分离鉴定及系统发育分析[J].中国兽医科学,2017,47(3):286-294,9.基金项目
国家科技支撑计划项目(2015BAI07B02-02) （2015BAI07B02-02）
黑龙江省自然科学基金重点项目(ZD2016006) （ZD2016006）
