中国兽医科学2017，Vol.47Issue(3)：295-300,6.DOI:10.16656/j.issn.1673-4696.2017.03.004
贵阳动物园动物隐孢子虫的调查与虫种鉴定
Investigation and identification of Cryptosporidium from animals in Guiyang Zoo
摘要
Abstract
In order to figure out the occurrence of Cryptosporidium in captive wild animals kept in Guiyang Zoo of Guizhou Province,we examined 84 fecal samples from camels,black bears,zebras,kangaroos,rhesus monkeys,gibbons and elephants.Sheather's sucrose flotation technique was used to collect oocysts and primers were designed for nested PCR amplification of 18S rRNA of Cryptosporidium.Finally,PCR products were examined by 10 g/L agarose gel electrophoresis and the positive products were sequenced and a phylogenetic tree was constructed.In result,there were 13 positive samples for Cryptosporidium.Seven camels were infected with C.andersoni and the infection rate was 70％.A zebra was infected with C.andersoni with the infection rate of i0％.Five gibbons feces with an infection rate of 23.81％ were Cryptosporidium-positive,and C.parvum,C.hominis and C.muris were identified.The relatively high prevalence(15.5％) and the occurrence of four zoonotic Cryptosporidium species have a public significance.This is the first report of zebras infected with Cryptosporidium in China.关键词
隐孢子虫/18S rRNA/巢式PCR/进化树/野生动物Key words
Cryptosporidium/18S rRNA/nested PCR/phylogenetic tree/wild animal分类
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宋源,肖莉,赵若璇,唐永平,谭永红,李兵,吴孔菊,彭广能,李威,钟志军,彭家兰,谢娜,黄祥明,邓磊,刘学涵,兰景超..贵阳动物园动物隐孢子虫的调查与虫种鉴定[J].中国兽医科学,2017,47(3):295-300,6.基金项目
成都大熊猫繁育研究基金会项目(CPF2014-14) （CPF2014-14）
国家自然科学基金项目(31272620) （31272620）
