中国兽医科学2017，Vol.47Issue(3)：309-314,6.DOI:10.16656/j.issn.1673-4696.2017.03.006
PADV3自然感染猪组织中病毒含量的实时荧光定量PCR检测
Detection of porcine adenovirus type 3 in the naturally infected pigs by real-time quantitative PCR
摘要
Abstract
In order to investigate the distribution of PADV3 in the naturally infected pigs,the realtime quantitative PCR was used to detect the virus content of PADV3 in different organs and tissues of piglets,sows and the pigs mixedly infected(PCV2) with natural infection.In result,PADV3 was detected in most organs but the virus content was different in different organs.The content was higher in the intestinal mucosa,lung,lymphoglandulae mesentericae and tonsil,but it was lower in the heart,liver and spleen.The content of PADV3 of the lung,lymphoglandulae mesentericae and tonsil in piglets mixedly infection with of PCV2 was significantly increased.For the intestinal mucosa,lung,lymphoglandulae mesentericae and tonsil,PADV3 was stronger in tropism,but was lower to the heart,liver,spleen.The existence of PCV2 in fection increased the proliferation and replication,and enhanced the pro-infection tissue tropism of PADV3.关键词
猪腺病毒3型/猪圆环病毒2型/实时荧光定量PCRKey words
porcine adenovirus type 3(PADV3)/PCV2/real-time quantitative PCR分类
农业科技引用本文复制引用
吕雯婷,方和俊,赵军,李萍,许思遥,杨凡,徐志文,朱玲..PADV3自然感染猪组织中病毒含量的实时荧光定量PCR检测[J].中国兽医科学,2017,47(3):309-314,6.基金项目
四川省科技支撑计划项目(2014NZ0043) （2014NZ0043）
教育部“长江学者与创新团队发展计划”项目(IRT13083) （IRT13083）
四川省“十三五”育种攻关项目(2016NYZ0052) （2016NYZ0052）
国家“十二五”科技支撑计划(2015BAD12B04-2.3) （2015BAD12B04-2.3）
