中国兽医科学2017，Vol.47Issue(3)：352-359,8.DOI:10.16656/j.issn.1673-4696.2017.03.013
胸膜肺炎放线杆菌血清7型菌株体内外培养转录差异的研究
Study on difference of transcription of Actinobacillus pleuropneumoniae serovar 7 strain in vitro and in vivo
摘要
Abstract
To reveal the transcriptional differences of Actinobacillus pleuropneumoniae serotype 7 cultured in vivo and in vitro,the bacteria were cultured to a logarithmic phase and intranasally inoculated to SPF Kunming mice.The lung tissue of the infected mouse and pure cultured bacteria were used for transcriptome sequencing (RNA-Seq),and the differences of transcription of the bacteria that cultured in vivo and in vitro by Illumina were analyzed.The results showed that,the alignment efficiency of the bacterial clean reads from the lung tissue of mouse was 15.68 ％,compared with the reference genome.As the transcriptome of the latter was the control,a total of 333 differentially expressed genes (DEG) were filtered out,of which 113 were up-regulated and 220 were down regulated,and 6 were new genes.GO,COG and KEGG analysis of DEG showed that these DEG were mainly enriched in"energy production and conversion","inorganic ion transport and metabolism","amino acid transport and metabolism",et al.These DEG were also mainly enriched in pathways like carbon metabolism,ABC transporters,purine metabolism,biosynthesis of amino acids,sulfur metabolism,et al.The current study on the transcriptional difference of A.pleuropneumoniae serotype 7 cultured in SPF Kunming mouse revealed the pathogenic mechanism of the bacteria to a certain extent,and provided some bases for future studies on the pathogenic mechanisms.关键词
胸膜肺炎放线杆菌/转录组学/差异基因Key words
Actinobacillus pleuropneumoniae/transcriptomics/DEG分类
农业科技引用本文复制引用
李萍,樊毅,殷玥,赵军,黄剑波,徐志文,朱玲..胸膜肺炎放线杆菌血清7型菌株体内外培养转录差异的研究[J].中国兽医科学,2017,47(3):352-359,8.基金项目
四川省科技支撑计划项目(2014NZ0043) （2014NZ0043）
教育部“长江学者与创新团队发展计划”项目(IRT13083) （IRT13083）
四川省十三五育种攻关项目(2016NYZ0052) （2016NYZ0052）
国家“十二五”科技支撑计划(2015BAD12B04-2.3) （2015BAD12B04-2.3）
