中国兽医科学2017，Vol.47Issue(3)：360-365,6.DOI:10.16656/j.issn.1673-4696.2017.03.014
伪狂犬病病毒和猪圆环病毒2型混合人工感染仔猪排毒的检测
Detection of dynamic changes of PRV and PCV2 loading and shedding
摘要
Abstract
In order to determine the dynamics of pseudorabies virus(PRV)and porcine circovirus type 2(PCV2)loading and shedding,34 healthy piglets were divided into 4 groups in this study.The groups 1,2 and 3 were inoculated with PRV,PCV2 and PRV+PCV2,respectively,while group 4 was set as control group.The nasal swab was collected and tested for viruses on the 3rd,7th,14th,21th and 35th days after inoculation.The qPCR method was used for quantitative detection of the changes of viruses.In result,PRV and PCV2 were detected from anal swab and nasal swab of the piglets in the 3 experimental groups on 3rd day post-inoculation.In the PRV infection group,the viral load reached the peak of 331.67 copies/μL on 14th day and in the PCV2 infection group,reached a peak value of i0 742.01 copies/μL on the 3rd day,after inoculation.PRV reached the peak of 980.96 copies/μL and 2 230.056 copies/μ L on the 14th and 7th day and PCV2 reached the peak of 69.413 and 271.72 copies/μL on the 14th day after inoculation.The piglets died from 8th to 14th day post-inoculation in the PCV2+PRVco-infection group.The level of PRV and PCV2 in the anal swab and the nasal swab were significantly different among the 3 experimental groups (P＜0.05).In conclusion,both the PRV or PCV2 single infection and the co-infection seriously harm the piglets,lead the death of piglets and result in shedding the 2 viruses through respiratory tract and digestive tract.关键词
仔猪/伪狂犬病病毒/猪圆环病毒2型/鼻拭子/肛门拭子/排毒规律Key words
piglet/pseudorabies virus (PRV)/porcine circovirus type 2 (PCV2)/nasal swab/anal swab/shedding分类
农业科技引用本文复制引用
宋春莲,颜洪奎,舒相华,张应国,李永能,尹革芬,李文贵,段博芳,耿孝杰,张雪,凌万旭..伪狂犬病病毒和猪圆环病毒2型混合人工感染仔猪排毒的检测[J].中国兽医科学,2017,47(3):360-365,6.基金项目
云南省科技惠民计划项目(2013CH001) （2013CH001）
昆明市科技合作重点项目(20130201AH023027) （20130201AH023027）
