中国畜牧杂志2017，Vol.53Issue(3)：33-39,7.DOI:10.19556/j.0258-7033.2017-03-033
MC1R基因在不同毛色太行山羊皮肤组织中的表达
The Expression of MC1R Gene in the Skin Tissues of Tai-hang Goat with Different Coat Colors
摘要
Abstract
This study aims to research the mRNA expression rules of Tai-hang goat MC1R gene in skin tissues with different colors.The coding sequence (CDS) of MC1R gene was amplified from skin tissues of Tai-hang goat with pure black color using reverse transcription polymerase chain reaction (RT-PCR).Structural characteristics of coded protein were analyzed by bioinformatics methods.Sixteen Tai-hang goats with four kinds of coat colors (black,white,brown and white with black-spots,respectively) were chosen to study the mRNA expression in skin tissues using quantitative realtime PCR.Results showed that the length of complete CDS was 954 bp encoding a protein with 317 amino acids,and the encoded protein is a member of G protein-coupled receptor family.Results of homology alignment and evolutionary tree showed its high homologies with sheep,cattle and buffalo.MC1R mRNA showed the highest expression in pure black skin tissues,followed by that in brown skin tissues.These results laid a foundation for further study on the role of MC1R gene in the formation of coat colors,and the pure breeding of black Tai-hang goat.关键词
太行山羊/MC1R基因/序列特征/mRNA表达Key words
Tai-hang goat/MC1R/Sequence characteristics/mRNA expression分类
农业科技引用本文复制引用
景炅婕,贾秀生,梁琛,乔利英,潘洋洋,刘建华..MC1R基因在不同毛色太行山羊皮肤组织中的表达[J].中国畜牧杂志,2017,53(3):33-39,7.基金项目
山西省科技攻关项目(20120312010-2) （20120312010-2）
科技部863子课题(2011AA100307-05) （2011AA100307-05）
山西省研究生优秀创新项目(2015SY27) （2015SY27）
