中国畜牧杂志2017，Vol.53Issue(3)：40-44,5.DOI:10.19556/j.0258-7033.2017-03-040
不同发育阶段延边黄牛阉牛背最长肌差异表达基因的筛选
Screening and Identification of the Differentially Expressed Genes in Different Development Stages of Yanbian Steers Longissimus Dorsi Muscles
摘要
Abstract
Annealing control primer (ACP) system was applied to find differentially expressed genes(DEGs) in the longissimus dorsi muscle between 8 and 30 months of age.Using 20 arbitrary ACP primers,we identified and sequenced 9 DEGs.The expression patterns were confirmed for five genes (Telethonin,MYH7,MSTN,Camk2d and SPP1) using realtime PCR.The expressions of MYH7,MSTN and SPP1 were higher in the 8 months old than that in 30 months (P＜0.05),The expressions of the Camk2d and Telethonin were higher in the 30 months than that in 8 months (P＜0.05).All the DEGs were screened by ACP system,which may considered as the most important genes involved in growth and meat quality traits.关键词
延边黄牛/引物退火控制技术/差异表达基因/荧光定量PCRKey words
Yanbian yellow cattle/Annealing control primer (ACP) system/Differential expression genes(DEGs)/Real-time quantitative PCR分类
农业科技引用本文复制引用
田万年,李香子,夏广军,金鑫,严昌国..不同发育阶段延边黄牛阉牛背最长肌差异表达基因的筛选[J].中国畜牧杂志,2017,53(3):40-44,5.基金项目
吉林省科技发展计划项目(20150623004TC) （20150623004TC）
吉林省重点学科培育项目(吉农院合字[2015]第x030号) （吉农院合字[2015]第x030号）
吉林农业科技学院种子基金项目(吉农院合字[2014]第Z07号) （吉农院合字[2014]第Z07号）
