中国畜牧杂志2017，Vol.53Issue(3)：58-62,5.DOI:10.19556/j.0258-7033.2017-03-058
BCB和Zebularine对绵羊体细胞核移植胚胎发育效果的影响
Effect of Brilliant Cresyl Blue Staining and Zebularine on Development of Somatic Cell Nuclear Transfer Embryos in Sheep
摘要
Abstract
To investigate whether oocytes selecting were favorable for the development of somatic cell nuclear transfer embryos in vitro by brilliant cresyl blue (BCB) staining,the cumulus-oocyte complexes (COCs) were stained in PBS containing BCB.According to the color of cytoplasm,the oocytes were divided into two groups:BCB+ group and BCB-group.The COCs without BCB treatment were used as the control group,then the oocytes were maturated in vitro and the maturation rate of oocytes was counted.The matured oocytes were then subjected to somatic cell nuclear transfer.The cumulus cells treated with Zebularine were used as the donor cells of some BCB+ oocytes.The cleavage rate,morula rate and blastocyst rate were counted.Results showed that the maturation rate of BCB+ group was significantly higher than that of control group and BCB group (71.15％ vs 65.38％,53.52％,P＜0.05);after somatic cell nuclear transfer,the development level of cloned embryos was evaluated,there is significant difference in the cleavage rate (87.91％ vs 56.83％),morula rate (37.41％ vs 21.73％) and blastocyst rate (21.48％ vs 6.82％) between BCB+ group and BCB group (P＜0.05).Compared with the control group,the cleavage rate (87.91％ vs 83.23％) and blastocyst rate (21.48％ vs 14.89％) ofBCB+ group were also significantly higher (P＜0.05).When donor cells were treated with Zebularine,the blastocyst rate of the BCB+ group (Zeb+BCB+ group) was further increased,it was significantly higher than that of BCB+ group,control group and BCB-group (29.25％ vs 21.48％,14.89％,6.82％,P＜0.05).关键词
BCB染色/Zebularine/绵羊/体细胞核移植Key words
Brilliant cresyl blue (BCB) staining/Zebularine/Sheep/Somatic cell nuclear transfer分类
农业科技引用本文复制引用
曹慧,李俊杰,郝永兰,刘爱菊,李文瑞,魏巧莉,田树军,孙树春..BCB和Zebularine对绵羊体细胞核移植胚胎发育效果的影响[J].中国畜牧杂志,2017,53(3):58-62,5.基金项目
河北省自然科学基金(C2014204119) （C2014204119）
河北省高等学校科学技术研究项目(ZD2014002) （ZD2014002）
