中国药房2017，Vol.28Issue(9)：1225-1228,4.DOI:10.6039/j.issn.1001-0408.2017.09.20
蒲黄中1种新染色掺假色素的确认及9种橙黄色色素的检测方法研究
Identification of a New Adulterated Dye and Study on Detection Method for 9 Orange-yellow Dyes in Typhae Pollen
摘要
Abstract
OBJECTIVE:To identify a new adulterated dye,and to establish simultaneous detection method for 9 orange-yel-low dyes in Typhae Pollen. METHODS:TLC and LC-MS were used for the identification of the new adulterated dye;TLC and HPLC method were used for determining 9 orange-yellow dyes. The determination was performed on Welch AQ-C18 column with mobile phase consisting of acetonitrile-0.05 mol/L ammonium acetate solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength were set at 432 nm (lemon yellow,metanil yellow,gold orange O,basic orangeⅡ) and 484 nm (sun-set yellow,acid orange Ⅰ,gold orangeⅡ,orange G,basic orange 21). The column temperature was 35 ℃.The sample size was 10 μL. RESULTS:TLC spots of auramine O,lemon yellow and new adulterated dye were clear and well separated without in-terference from negative control;new adulterated dye was identified as metanil yellow. TLC spots of lemon yellow,sunset yellow, orange G,acid orangeⅠ,gold orangeⅡ,auramine O,metanil yellow,basic orange 21 and basic orangeⅡwere clear and well sep-arated without interference from negative control. The limits of detection were 0.30,0.20,0.33,0.16,0.19,0.19,0.31,0.26, 0.30 mg/kg. CONCLUSIONS:The established method is able to detect adulterated dye and orange-yellow dye in Typhae Pollen rap-idly and accurately.关键词
蒲黄/色素/薄层色谱法/液相色谱-质谱联用法/高效液相色谱法Key words
Typhae Pollen/Dye/TLC/LC-MS/HPLC分类
医药卫生引用本文复制引用
耿昭,张燕飞,苟琰,周娟,郭力,吴强..蒲黄中1种新染色掺假色素的确认及9种橙黄色色素的检测方法研究[J].中国药房,2017,28(9):1225-1228,4.基金项目
国家科技重大专项课题(No.2014ZX09304307-002) （No.2014ZX09304307-002）
