中国药房2017，Vol.28Issue(9)：1231-1235,5.DOI:10.6039/j.issn.1001-0408.2017.09.22
精源胶囊的质量标准研究
Study on Quality Standard of Jingyuan Capsules
摘要
Abstract
OBJECTIVE:To establish the quality standard of Jingyuan capsule. METHODS:TLC was used for qualitative iden-tification of Epimedium brevicornu,Pseudostellaria heterophylla and Fallopia multiflora in the preparation. HPLC method was used to determine the content of icariin. The determination was performed on Odyssil C18 column with mobile phase consisting of acetoni-trile-water(28:72,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 270 nm,and the column temperature was 30 ℃. The sample size was 10 μL. The contents of polysaccharides was determined by phenol-sulfuric method. RESULTS:TCL spots of E. brevicornu,P. heterophylla and F. multiflora were clear and well separated without interference from negative con-trol. The linear range of icariin ranged 0.027-0.135 mg/mL(r=0.9999);RSDs of precision,stability and repeatability test were all lower than 2.0%. The recoveries were 97.87%-101.94%(RSD=1.47%,n=9). The linear range of glucose ranged 0.056-0.121 mg/mL(r=0.9995),and RSDs of precision,stability and repeatability tests were all lower than 2.0%. The recoveries were 99.37%-100.38%(RSD=0.36%,n=6). CONCLUSIONS:The established standard can be used for the quality control of Jingyuan capsule.关键词
精源胶囊/质量标准/薄层色谱法/淫羊藿苷/多糖/高效液相色谱法Key words
Jingyuan capsule/Quality standard/TLC/Icariin/Polysaccharide/HPLC分类
医药卫生引用本文复制引用
段丽,张永萍,徐剑,迮薇薇,闻家政,钟敬根..精源胶囊的质量标准研究[J].中国药房,2017,28(9):1231-1235,5.基金项目
国家工程技术研究中心组建项目(No.2014F M 125Q09) （No.2014F M 125Q09）
贵州省中药现代化科技产业研究开发专项项目(No.黔科合中药字〔2013〕5009) （No.黔科合中药字〔2013〕5009）
贵州省普通高等学校工程研究中心建设项目(No.黔教合KY字〔2014〕22号) （No.黔教合KY字〔2014〕22号）
贵州省高层次创新型人才培养项目(No.黔科合人才〔2015〕4030号) （No.黔科合人才〔2015〕4030号）
