中国药房2017，Vol.28Issue(9)：1236-1238,3.DOI:10.6039/j.issn.1001-0408.2017.09.23
高效阳离子交换色谱法测定尿素[13C]胶囊中尿素的含量
Content Determination of Urea in Urea [13C] Capsules by High Performance Cation-exchange Chromatogra-phy
摘要
Abstract
OBJECTIVE:To determine the content of urea in Urea [13C] capsules by high performance cation-exchange chroma-tography (HPCEC). METHODS:The determination was performed on Zorbax 300 SCX column with mobile phases consisting of acetonitrile-0.1% phosphoric acid (20:80,V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 200 nm and column temperature was 35 ℃. The sample size was 20 μL. RESULTS:The linear range of urea was 0.0039-1.0030 mg/ml(r=0.9997). The limit of quantitation was 3.918 μg/mL and the limit of detection was 0.975 μg/mL. RSDs of precision,stability and repetitive test were all lower than 2.0%. The recovery ranged 99.3%-101.0%(RSD=0.67%,n=9). CONCLUSIONS:The meth-od is simple,rapid,sensitive and suitable for the content determination of urea in Urea [13C] capsules.关键词
尿素[13C]胶囊/尿素/含量测定/阳离子交换法Key words
Urea [13C] capsules/Urea/Content determination/High performance cation-exchange chromatography分类
医药卫生引用本文复制引用
刘桂霞,姚静,左利民,李盼盼,山广志,姜威..高效阳离子交换色谱法测定尿素[13C]胶囊中尿素的含量[J].中国药房,2017,28(9):1236-1238,3.基金项目
国家自然科学基金资助项目(No.81573474) （No.81573474）
中国医学科学院医学与健康科技创新工程系统创新团队基金(No.2016-I2M-3-010) （No.2016-I2M-3-010）
