中国药房2017，Vol.28Issue(9)：1239-1242,4.DOI:10.6039/j.issn.1001-0408.2017.09.24
HPLC法同时测定茵胆平肝胶囊中6种成分的含量
Simultaneous Determination of 6 Kinds of Components in Yindan Pinggan Capsules by HPLC
摘要
Abstract
OBJECTIVE:To develop a method for simultaneous determination of chlorogenic acid,geniposide,gentiopicro-side,ferulic acid,baicalin and ammonium glycyrrhizinate in Yindan pinggan capsule. METHODS:HPLC method was adopted. The separation was performed on Wonda SilTM-C18 column with mobile phase consisting of acetonitrile-0.4% phosphoric acid solu-tion(gradient elution)at the flow rate of 0.8 mL/min. The detection wavelength were set at 325 nm(chlorogenic acid,ferulic ac-id),250 nm (geniposide,ammonium glycyrrhizinate) and 275 nm (gentiopicroside,baicalin). The column temperature was 30 ℃. RESULTS:The linear ranges were 0.087-3.480 μg for chlorogenic acid(r=0.9998),0.201-8.040 μg for geniposide(r=0.9997),0.200-8.000 μg for gentiopicroside(r=0.9995),0.016-0.640 μg for ferulic acid(r=0.9999),0.105-4.200 μg for ba-icalin (r=0.9999) and 0.028-1.120 μg for ammonium glycyrrhizinate (r=0.9995),respectively. The limits of quantitation were 1.31,0.75,1.14,1.25,0.94,0.98 ng,and the limits of detection were 0.87,0.67,0.96,0.93,0.60,0.88 ng,respectively. RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 99.9%-101.9%(RSD=0.7%,n=6), 98.7%-100.9%(RSD=0.9%,n=6),98.1%-101.5%(RSD=1.4%,n=6),98.5%-101.3%(RSD=1.3%,n=6),98.5%-101.7%(RSD=1.2%,n=6),98.2%-101.4%(RSD=1.2%,n=6),respectively. CONCLUSIONS:The method is simple and accurate , can be used for simultaneous determination of chlorogenic acid,geniposide,gentiopicroside,ferulic acid,baicalin and ammonium glycyrrhizinate in Yindan pinggan capsule.关键词
茵胆平肝胶囊/绿原酸/栀子苷/龙胆苦苷/阿魏酸/黄芩苷/甘草酸铵/高效液相色谱法/含量测定Key words
Yindan pinggan capsule/Chlorogenic acid/Geniposide/Gentiopicroside/Ferulic acid/Baicalin/Ammonium glyc-yrrhizinate/HPLC/Content determination分类
医药卫生引用本文复制引用
张宏梅,昌盛,崔佰吉,李星梅,冯宪敏..HPLC法同时测定茵胆平肝胶囊中6种成分的含量[J].中国药房,2017,28(9):1239-1242,4.基金项目
国家自然科学基金资助项目(No.31572262) （No.31572262）
吉林省科技发展计划项目(No.20160414050GH) （No.20160414050GH）
