中国药房2017，Vol.28Issue(9)：1242-1245,4.DOI:10.6039/j.issn.1001-0408.2017.09.25
微流控芯片非接触电导法测定异烟肼片中异烟肼的含量
Content Determination of Isoniazid in Isoniazid Tablets by Microfluidic Chip with Contactless Conductivi-ty Method
摘要
Abstract
OBJECTIVE:To establish a method for the content determination of isoniazid in Isoniazid tablet. METHODS:Mi-crofluidic chip with contactless conductivity detection was adopted. The effects of electrophoresis parameters,such as the variety, concentration,pH,additive types and dosage,injection time and separation voltage on the separation detection were explored. RE-SULTS:The optimized buffer system was 1.86 mmol/L citrate-0.14 mmol/L sodium citrate buffer solution (pH3.0),no additive , the injection time was 20 s and the separation voltage was 2.5 kV. In the optimal conditions,the linear range of isoniazid was 10-200 μg/mL(r=0.9993);the limits of quantification and detection were 8.0 μg/mL and 2.4 μg/mL;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 97.8%-102.9%(RSD=1.9%,n=9). CONCLUSIONS:The method is rapid and simple,and suitable for the content determination of isoniazid in Isoniazid tablet.关键词
微流控芯片/非接触电导检测/异烟肼/异烟肼片/含量测定Key words
Microfluidic chip/Contactless conductivity detection/Isoniazid/Isoniazid tablet/Content determination分类
医药卫生引用本文复制引用
童艳丽,冯汗志,陈缵光..微流控芯片非接触电导法测定异烟肼片中异烟肼的含量[J].中国药房,2017,28(9):1242-1245,4.基金项目
广东省科技计划项目(No.2014A020221098) （No.2014A020221098）
