中国药房2017，Vol.28Issue(9)：1246-1249,4.DOI:10.6039/j.issn.1001-0408.2017.09.26
HPLC法同时测定龙泽熊胆胶囊中6种成分的含量
Simultaneous Determination of 6 Kinds of Components in Longze Xiongdan Capsules by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of chlorogenic acid,geniposide,gentiopicro-side,baicalin,berberine hydrochloride and chrysophanol in Longze xiongdan capsule. METHODS:HPLC method was adopted. The separation was performed on Dikma Platisil ODS column with mobile phase consisting of acetonitrile-0.1% phosphoric acid so-lution(gradient elution)at the flow rate of 1.0 mL/min. The detection wavelength were set at 254 nm for geniposide,gentiopicro-side,baicalin,berberine hydrochloride and chrysophanol and 327 nm for chlorogenic acid. The column temperature was 30 ℃. RE-SUITS:Linear ranges of chlorogenic acid,geniposide,gentiopicroside,baicalin,berberine hydrochloride and chrysophanol were 0.0082-0.164 μg (r=0.9998),0.027-0.540 μg(r=0.9997),0.02525-0.505 μg(r=0.9998),0.046-0.920 μg(r=0.9997), 0.059-1.180 μg(r=0.9995),0.00805-0.161 μg(r=0.9994),respectively;the limits of quantitation were 0.61,0.59,0.87,0.51, 0.92,0.65 ng,and the limits of detection were 1.67,1.73,1.96,1.82,2.31,1.87 ng. RSDs of precision,stability and reproduc-ibility tests were lower than 2.0%;recoveries were 95.34%-98.27%(RSD=1.2%,n=9),98.18%-101.92%(RSD=1.4%,n=9), 97.36% -101.09%(RSD=1.0% ,n=9),95.24% -98.93%(RSD=1.3% ,n=9), 95.76% -99.34%(RSD=1.5% ,n=9), 95.00%-98.75%(RSD=1.4%,n=9),respectively. CONCLUSIONS:The method is simple,accurate,reproducible and suitable for simultaneous determination of chlorogenic acid,geniposide,gentiopicroside,baicalin,berberine hydrochloride,chrysophanol in Longze xiongdan capsule.关键词
高效液相色谱法/龙泽熊胆胶囊/绿原酸/栀子苷/龙胆苦苷/黄芩苷/盐酸小檗碱/大黄酚/含量Key words
HPLC/Longze xiongdan capsule/Chlorogenic acid/Geniposide/Gentiopicroside/Baicalin/Berberine hydrochlo-ride/Chrysophanol/Content分类
医药卫生引用本文复制引用
郝乘仪,冯波..HPLC法同时测定龙泽熊胆胶囊中6种成分的含量[J].中国药房,2017,28(9):1246-1249,4.基金项目
吉林省卫生科研计划项目(No.2013Z008) （No.2013Z008）
