中国药房2017，Vol.28Issue(9)：1265-1267,3.DOI:10.6039/j.issn.1001-0408.2017.09.31
东北地区铁线透骨草药材的HPLC指纹图谱研究
Study on the HPLC Fingerprints of Herba clematidis in Northeast
摘要
Abstract
OBJECTIVE:To establish the HPLC fingerprints for Herba clematidis in northeast. METHODS:HPLC was per-formed on the column of Hedera ODS-2 C18 with mobile phase of acetonitrile-0.5% phosphoric acid solution(gradient elution)at a flow rate of 1.0 mL/min,detection wavelength was 338 nm,column temperature was 30 ℃,and the injection volume was 20 μL. Using rutin as as a reference,the HPLC profiles of 10 batches of H. clematidis were determined,Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine(2004A edition) was used for the common peaks identification and similarity evaluation. RESULTS:There were 16 common peaks in the 10 batches of H. clematidis,similarity degree was higher than 0.9. It was proved that the HPLC profiles and control fingerprint profile of 10 batches of H. clematidis had good consistency. CONCLUSIONS:The established fingerprints can provide reference for the identification and quality evaluation of H. clematidis in northeast.关键词
东北地区/铁线透骨草/高效液相色谱法/指纹图谱Key words
Northeast/Herba clematidis/HPLC/Fingerprint chromatogram分类
医药卫生引用本文复制引用
贾媛,张恒玉,柴尧,彭九嫚,杜俊潮..东北地区铁线透骨草药材的HPLC指纹图谱研究[J].中国药房,2017,28(9):1265-1267,3.基金项目
南京市江宁区科技项目(No.2015Eg04) （No.2015Eg04）
