中国肿瘤生物治疗杂志2017，Vol.24Issue(3)：230-236,7.DOI:10.3872/j.issn.1007-385X.2017.03.003
γδT细胞对血液肿瘤细胞的体外杀伤活性
Killing activity in vitro of γδT cells against human hematologic neoplasms cells
摘要
Abstract
Objective:To establish culture system of γδT cell and to explore killing activity of the γδT cells against different human hematologic neoplasms cells.Methods:Four patients with lymphoma who were hospitalized in the Department of Hematopoietic Stem Cell Transplantation,the 307th Hospital of PLA and five healthy volunteers for physical examination during January to April 2016 were selected and their peripheral blood mononuclear cell (PBMC) were isolated respectively.γδT cells were amplified in vitro with zoledronate (Zol) and IL-2,and killing activities of the γδT cells against hematologic neoplasms Jurkat,THP-1,HL-60,K562,Raji,U-937 and RPMI-8226 line cells were investigated by Flow cytometry assay.Killing effects of CIK cells,NK cells and the γδT cells on K562 cells were compared;and the expression levels of IFN-γ,TNF-α and secretion level of CD107a molecule in the γδT cells were detected.Results:The γδT cells were successfully amplified from peripheral blood in vitro.The γδT cells showed obvious killing activities against all of the Jurkat,THP-1,HL-60,K562,U-937 and RPMI-8226 cells (P ＜ 0.05).There was not a statistical difference between killing activities of the γδT and NK cells against the K562 cells (P ＞ 0.05),but killing activity of the γδT cells against K562 cells was higher than that of CIK cells (P ＜ 0.01).With extending the co-incubation time with K562 cells,level of IFN-γsecreted by the γδT cells increased as a time-dependent manner,in addition,level of TNF-α in the γδT cells gradually increased after 8 h of the co-incubation.After co-incubation of the γδT cells with the K562 or the HL-60 cells,expression level of CD107a molecule in the γδT cells was significantly up-regulated (P ＜ 0.01).Conclusion:The γδT cells amplified showed higher killing activity against hematologic neoplasms cells,which could provide experimental evidence for cellular immunotherapy of hematologic neoplasms.关键词
γδT细胞/血液肿瘤细胞/细胞免疫治疗Key words
γδT cell/hematologic neoplasms cells/cellular immunotherapy分类
医药卫生引用本文复制引用
肖凌,张斌,陈虎..γδT细胞对血液肿瘤细胞的体外杀伤活性[J].中国肿瘤生物治疗杂志,2017,24(3):230-236,7.基金项目
国家高技术研究发展计划(863计划)资助项目(2013AA0200103) （863计划）
北京市科委首都特色课题基金资助项目(Z161100000516184). Project supported by the National High Technology Research and Development Program(863 program) of China (2013AA0200103),and the Beijing Municipal Science and Technology Characteristics of the Capital Project Foundation (Z161100000516184) （Z161100000516184）
