中国肿瘤生物治疗杂志2017，Vol.24Issue(3)：264-270,7.DOI:10.3872/j.issn.1007-385X.2017.03.009
siRNA干扰HMGB1表达对膀胱癌T24株细胞恶性生物学行为的影响及其可能的机制
Effects of siRNA interfering expression of HMGB1 on malignant biological behaviors of bladder cancer T24 line cell and its possible mechanism
摘要
Abstract
Objective:To explore effects of high-mobility group box 1 (HMGB1) on proliferation,apoptosis and malignant biological behaviors of bladder cancer T24 cell line as well as its potential mechanism.Methods:Twenty cases of surgically resected bladder cancer and corresponding adjacent normal tissues form the patients who were hospitalized in Department of Urinary Surgery,the 1 st Hospital affiliated to Chongqing Medical University during December 2014 to January 2016 were collected.The T24 cells were treated by RNAi technique and divided into blank control group,negative control (NC) group and interfere (siHMGB1) group.Differences of HMGB1 expression between bladder cancer and adjacent normal tissues were detected by immuno chemistry assay.CCK-8 assay,flow cytometry assay,scratch test and Transwell invasion test were used to examine effect of knocking down HMGB1 on abilities of proliferation,apoptosis,cell cycle,migration and invasion of the T24 cells respectively.Western blotting assay was used to detect expression levels of HMGB1 in BIU-87 and T24 cells and effect of knocking down HMGB1 on expression of malignant biological behavior related proteins in the T24 cells.Results:Expression of HMGB1 in the bladder cancer tissues was obviously higher than that in the adjacent normal tissues ([67.33 ± 4.91] vs [12.00 ± 3.79],P ＜ 0.05).Comparing with the negative control and blank control groups,proliferation of the T24 cells was inhibited in the siHMGB1 group (P ＜0.05).Results of flow cytometry assay suggested that apoptosis rate of the T24 cells increased and its cell cycle arrested in phase of G0/G1 after knocking down HMGB1;results of scratch and Transwell invasion tests showed that after knock-down of HMGB1,abilities of migration (P ＜0.05) and invasion ([16.33 ±1.45] vs [35.00 ±1.53],[34.00 ±2.08],P＜0.05] of the T24 cells decreased.Results of Western blotting assay showed that after the knock-down,expression of E-cardherin protein was upregulated,expressions of N-cadherin,vimentin,metrix metalloproteinase(MMP)-2,MMP-9,cyclinD1,c-Myc and β-catenin proteins were down-regulated (all P ＜ 0.05).Conclusion:HMGB1 could promote epithelial-mesenchymal transition (EMT) of the bladder cancer cells and then enhance their malignant biological behaviors.The mechanism might be mediated by β-catenin signaling pathway.关键词
高迁移率族蛋白1/膀胱癌细胞/上皮间质转化/β-联蛋白Key words
high-mobility group box 1 (HMGB1)/bladder cancer cell/epithelial-mesenchymal transition (EMT)/β-catenin分类
医药卫生引用本文复制引用
印胡滨,何卫阳,苟欣,谷文,刘言..siRNA干扰HMGB1表达对膀胱癌T24株细胞恶性生物学行为的影响及其可能的机制[J].中国肿瘤生物治疗杂志,2017,24(3):264-270,7.基金项目
国家自然科学基金资助项目(No.81372758).Project supported by the National Natural Science Foundation of China(No.81372758) （No.81372758）
