中国肿瘤生物治疗杂志2017，Vol.24Issue(3)：278-283,6.DOI:10.3872/j.issn.1007-385X.2017.03.011
基于Keap1基因多态性的肾透明细胞癌分子标志物的研究
Research on the molecular markers of clear cell renal cell carcinoma based on polymorphisms of the Keap1 gene
李燕妮 1齐士勇 2颜艳 1何振 2徐勇 2邹慧儒1
作者信息
- 1. 天津市口腔医院暨南开大学口腔医院中心实验室,天津300041
- 2. 天津医科大学第二医院泌尿外科,天津300211
- 折叠
摘要
Abstract
Objective:To explore the correlation between single nucleotide polymorphism (SNP) of Keap1 gene and clear cell renal cell carcinoma (ccRCC),and to provide an effective molecular target for the clinical diagnosis and treatment of the ccRCC.Methods:One hundred and eight carcinoma tissues from the patients with ccRCC (tumor group) and 86 normal renal tissues from the tumor-free individuals (control group) were collected.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and gene sequencing assays were used to detect NSP of the Keap1 gene and genotype of the samples of the both groups.Expression status of the Keap1 gene in the both groups were examined by real-time fluorescence quantitative PCR.Results:Distribution frequencies of the Keap1 gene rs1048289 in different genotypes and alleles were statistically different between the tumor group and the control group (P ＜ 0.05).Risk of ccRCC in the patients carraying AA genotype and A allele obviously increased,and AA genotype (OR =2.292,95％ CI:1.159-4.533,P ＜ 0.05) and A allele (OR =2.067,95％ CI:1.280-3.342,P ＜ 0.01) were risk factors of ccRCC.Expression of the Keap1 gene in the patients of the tumor group was significantly lower than that in the patients of the control group (P ＜ 0.05).But there was no obvious difference in expression of the Keap1 gene among samples with different genotypes (P ＜ 0.05).Conclusion:The Keap1 gene rs1048289 could be significantly correlated with ccRCC,and could hopefullv become an effective molecular target for early diagnosis and gene therapy of the patients with ccRCC.关键词
单核苷酸多态性/肾透明细胞癌/分子标志物/早期诊断/基因治疗Key words
single nucleotide polymorphism (SNP)/clear cell renal cell carcinoma (ccRCC)/molecular marker/early diagnosis/gene therapy分类
医药卫生引用本文复制引用
李燕妮,齐士勇,颜艳,何振,徐勇,邹慧儒..基于Keap1基因多态性的肾透明细胞癌分子标志物的研究[J].中国肿瘤生物治疗杂志,2017,24(3):278-283,6.
