中成药2017，Vol.39Issue(3)：466-470,5.DOI:10.3969/j.issn.1001-1528.2017.03.005
五子衍宗方对环磷酰胺致小鼠睾丸细胞DNA损伤的影响
Effects of Wuzi Yanzong Prescription on cyclophosphamide-induced DNA damage of testis cells in mice
摘要
Abstract
AIM To investigate the effects of Wuzi Yanzong Prescription (Lycii Fructus,Cuscutae Semen,Rubi Fructus,Plantaginis Semen and Schisandrae chinensis Fructus) on DNA damage of testis cells in adult male mice induced by cyclophosphamide (CTX).METHODS Forty out of fifty adult male Balb/C mice were injected intraperitoneally with CTX and then were randomly and equally divided into model control group (normal saline),Wuzi Yanzong Prescription low-,medium-and high-dose groups (100,200 and 400 mg/kg),and the other ten mice served as normal control group (normal saline).All mice were anesthetized by inhalation of ether,and then were sacrificed by cervical dislocation.The sperm count,sperm motility and malformation rate of sperm were tested.The content of 8-hydroxy-deoxyguanosine (8-OHdG) in serum was measured using ELISA;the DNA damage degree of cells in testis was detected by single cell gel electrophoresis;the protein expressions of p-P53 and γ-H2AX in testis were examined by Western blot.RESULTS Compared with the model control group,Wuzi Yanzong Prescription groups showed significant increase in the sperm count,sperm motility and significantly decreased malformation rate of sperm,the level of 8-OHdG in serum,and the protein expressions of p-P53 and γ-H2AX in testis were also significantly decreased.CONCLUSION Wuzi Yanzong Prescription can significantly alleviate the DNA damage of testis cells in mice induced by CTX through down-regulating protein expressions of p-P53 and γ-H2AX.关键词
五子衍宗方/环磷酰胺/8-OHdG/DNA损伤/γ-H2AXKey words
Wuzi Yanzong Prescription/cyclophosphamide/8-OHdG/DNA damage/γ-H2AX分类
医药卫生引用本文复制引用
赵海霞,黄威峰,宋来新,刘静,彭奔,张长城..五子衍宗方对环磷酰胺致小鼠睾丸细胞DNA损伤的影响[J].中成药,2017,39(3):466-470,5.基金项目
国家自然科学基金项目(81573931,81373881,81503334) （81573931,81373881,81503334）
三峡大学人才科研启动基金(KJ2014B067) （KJ2014B067）
