中成药2017，Vol.39Issue(3)：471-475,5.DOI:10.3969/j.issn.1001-1528.2017.03.006
开心散对APP/PS1转基因小鼠在体LTP和PSD-95表达的影响
Effects of Kaixin Powder on LTP in vivo and expression of PSD-95 in APP/PS1 transgenic mice
摘要
Abstract
AIM To explore the effects of Kaixin Powder (Polygalae Radix,Ginseng Radix et Rhizoma,Poria and Acori tatarinowii Rhizoma) on hippocampal long-term potentiation (LTP) in vivo in APP/PS1 transgenic mice and expression of PSD-95 in hippocampus CA1 area and cerebral cortex.METHODS Thirty-two 3-monthold male APP/PS1 mice were randomly divided into model group,Donepezil [0.75 mg/(kg · d)] group,Kaixin Powder [1.5,3 g/(kg · d)] groups.C57/BL6 mice were used as the control group.Kaixin Powder and Donepezil were administered to the APP/PS1 mice for three months,respectively.Afterwards,the LTP in vivo was recorded in a perforant pathway-dentate gyrus in the hippocampus.Immunohistochemical method was used for measuring the expression of PSD-95 in hippocampus CA1 area and cerebral cortex.RESULTS The fEPSPs slopes were significantly decreased in the model group as compared with the control group.Kaixin Powder or Donepezil treatment significantly enhanced the fEPSPs slopes in the model group.The average optical density of PSD-95 positive cells in hippocampus CA1 area and cerebral cortex was obviously decreased in the model group.Donepezil increased the average optical density of PSD-95 positive cells in hippocampus CA1 area.Kaixin Powder [1.5 g/(kg · d)] increased the average optical density of PSD-95 positive cells in hippocampus CA1 area and cerebral cortex.Kaixin Powder [3 g/(kg · d)] increased the average optical density of PSD-95 positive cells in cerebral cortex.CONCLUSION Kaixin Powder can promote the LTP formation in APP/PS1 transgenic mice and enhance synaptic plasticity,which may be related to the regulation of protein expression of PSD-95.关键词
开心散/APP/PS1转基因小鼠/LTP/PSD-95Key words
Kaixin Powder/APP/PS1 transgenic mice/LTP/PSD-95分类
医药卫生引用本文复制引用
狄亚琪,桑旭星,方芳..开心散对APP/PS1转基因小鼠在体LTP和PSD-95表达的影响[J].中成药,2017,39(3):471-475,5.基金项目
国家自然科学基金(81373995) （81373995）
