中成药2017，Vol.39Issue(3)：528-532,5.DOI:10.3969/j.issn.1001-1528.2017.03.017
HPLC法同时测定银黄颗粒中6种成分
Simultaneous determination of six constituents in Yinhuang Granules by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid,caffeic acid,luteoloside,baicalein,luteolin and rutin in Yinhuang Granules (Lonicerae japonicae Flos and Scutellariae Radix extracts).METHODS The analysis of 80％ methanol extract of this drug was performed on a 30 ℃ thermostatic SunFire-C1scolumn (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile0.4％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 239 nm.RESULTS All the six constituents showed good linear relationships within their own ranges (r =0.999 9),whose average recoveries were 94.56％-98.38％ with the RSDs of 0.49％-2.89％.Among fourteen batches of samples,the luteoloside content was found to be of little difference,while the other five constituents' contents were of relatively great differences.CONCLUSION The qualities of Yinhuang Granules and Lonicerae japonicae Flos extracts from different manufacturers are uneven,so supervision should be strengthened.关键词
银黄颗粒/绿原酸/咖啡酸/木犀草苷/黄芩苷/木犀草素/芦丁/HPLCKey words
Yinhuang Granules/chlorogenic acid/caffeic acid/luteoloside/baicalein/luteolin/rutin/HPLC分类
医药卫生引用本文复制引用
艾光丽,王维,曾桢,蒲旭峰..HPLC法同时测定银黄颗粒中6种成分[J].中成药,2017,39(3):528-532,5.基金项目
2015年成都市食品安全风险研究项目(2015cdfx01) （2015cdfx01）
