中成药2017，Vol.39Issue(3)：532-535,4.DOI:10.3969/j.issn.1001-1528.2017.03.018
HPLC法同时测定降脂颗粒中5种成分
Simultaneous determination of five constituents in Jiangzhi Granules by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of five constituents in Jiangzhi Granules (Salviae miltiorrhizae Radix et Rhizoma,Polygoni cuspidati Rhizoma et Radix,Artemisiae scopariae Herba,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Hanbon-C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile (A) 0.1％ phosphoric acid (B) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 286 nm (0-30,40-50 min) and 203 nm (30-40 min).RESULTS Gypenoside A,chlorogenic acid,polydatin,salvianolic acid B and emodin showed good linear relationships within the ranges of 0.176-2.46,0.056-0.84,0.36-5.40,0.192-2.88 and 0.12-1.80 μg (r ＞0.999 0),respectively,whose average recoveries were 97.17％-102.59％ with the RSDs of 1.9％-2.9％.CONCLUSION This accurate and simple method can be used for the quality control of Jiangzhi Granules.关键词
降脂颗粒/绞股蓝皂苷A/绿原酸/虎杖苷/丹酚酸B/大黄素/HPLCKey words
Jiangzhi Granules/gypenoside A/chlorogenic acid/polydatin/salvianolic acid B/emodin/HPLC分类
医药卫生引用本文复制引用
马赟,王起文,徐国华,纪乐军,陈建伟,李祥..HPLC法同时测定降脂颗粒中5种成分[J].中成药,2017,39(3):532-535,4.基金项目
江苏省科技成果转化项目(BA2014118) （BA2014118）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
