中成药2017，Vol.39Issue(3)：551-555,5.DOI:10.3969/j.issn.1001-1528.2017.03.023
补肾强身片UPLC指纹图谱
UPLC fingerprints of Bushen Qiangshen Tablets
摘要
Abstract
AIM To establish the UPLC fingerprints of Bushen Qiangshen Tablets (Epimedii Folium,Cuscutae Semen,Rosae Laevigatae Fructus,etc.).METHODS The analysis of 50％ ethanol extract of this drug was performed on a 40 ℃ thermostatic Agilent SB-C18 column (100 mm ×4.6 mm,2.7 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelength was set at 215 nm.Then the fringerprints were evaluated by cluster analysis,principal components analysis and orthogonal partial least squares discriminant analysis.RESULTS There were twenty common peaks in the UPLC fingerprints of twelve batches of samples,nine of which (protocatechuic acid,salidroside,chlorogenic acid,hyperin,specnuezhenide,epimedin C,icariin,kaempferide and baohuoside Ⅰ) were identified,with the similarities of 0.843-0.970.Twelve batches of samples could be divided into three types,and four differential markers,including specnuezhenide and chlorogenic acid,were found out.CONCLUSION This simple and reliable method can be used for the quality control of Bushen Qiangshen Tablets.关键词
补肾强身片/UPLC指纹图谱/聚类分析/主成分分析/正交偏最小二乘判别分析Key words
Bushen Qiangshen Tablets/UPLC fingerprints/cluster analysis/principal components analysis/orthogonal partial least squares discriminant analysis分类
医药卫生引用本文复制引用
林秀莲,宋粉云,李华,黄寿辉..补肾强身片UPLC指纹图谱[J].中成药,2017,39(3):551-555,5.基金项目
上海市药物(中药)代谢产物研究重点实验室开放课题(2015SHDX1001) （中药）
