中成药2017，Vol.39Issue(3)：565-569,5.DOI:10.3969/j.issn.1001-1528.2017.03.026
11个品种来源陈皮中多甲氧基黄酮的测定
Determination of polymethoxylated flavones in Citri Reticulatae Pericarpium from eleven cultivar origins
摘要
Abstract
AIM To determine the contents of polymethoxylated flavones in Citri Reticulatae Pericarpium from eleven cultivar origins.METHODS HPLC was applied to screening extraction solvents (methanol,anhydrous ethanol,95％ ethanol,ethyl acetate and petroleum ether).Ultraviolet-visible spectrophotometry was adopted in the content determination of polymethoxylated flavones in eighteen batches of samples,nobiletin was taken as a reference substance,and the detection wavelength was set at 330 nm.RESULTS Ethyl acetate exhibited the best extraction effect,so it was selected as extraction solvent.Nobiletin showed a good linear relationship within the range of 3.024-13.104 μg/mL (R2 =0.999 8),whose average recovery was 101.37％ with the RSD of 1.34％.The contents of polymethoxylated flavones had obvious differences among samples from different cultivar origins,which were relatively high from Citrus reticulata ‘ Chachi’,Citrus reticulata ‘ Dahongpao’,Citrus reticulata ‘ Tangerina’,Citrus reticulata ‘ Ponkan’,Citrus reticulata ‘ Kinokuni’and Citrus reticulata ‘ Shiyueju’(more than 0.4％).CONCLUSION This simple and accurate method can effectively eliminate the interference of another flavonoids,which is suitable for the content determination of polymethoxylated flavones in Citri Reticulatae Pericarpium.关键词
陈皮/品种来源/多甲氧基黄酮/紫外-可见分光光度Key words
Citri Reticulatae Pericarpium/cultivar origins/polymethoxylated flavones/ultraviolet-visible spectrophotometry分类
医药卫生引用本文复制引用
罗琥捷,杨宜婷,李晓伟,罗美霞,胡坪君,陈浩,郑国栋..11个品种来源陈皮中多甲氧基黄酮的测定[J].中成药,2017,39(3):565-569,5.基金项目
国家自然科学基金青年基金项目(31401613) （31401613）
2014年广东省高校优秀青年培养计划项目[粤教师函(2014)145] （2014）
广州市属高校科研计划项目(2012C210) （2012C210）
2015年广州医科大学学生课外科研项目(2015B018) （2015B018）
