中国中西医结合杂志2017，Vol.37Issue(3)：319-325,7.DOI:10.7661/CJIM.2017.03.0319
小儿支气管哮喘发作期证候学标记物的代谢组学研究
Metabonomics of Syndrome Markers in Infantile Bronchial Asthma Episode
摘要
Abstract
Objective To analyze urinary metabolites of bronchial asthma children patients with phlegm-heat obstructing Fei syndrome (PHOFS) and non-PHOFS using gas chromatography-mass spectrometry/mass spectrometer (GC-MS/MS),thus performing research on syndrome markers.Methods Totally 44 bronchial asthma children patients with PHOFS and non-PHOFS in onset of asthma were recruited.Another 29 healthy children were also recruited.Their urine samples were analyzed by GC-MS/MS.The profiles were analyzed using orthogonal partial least squares-discriminant analysis (OPLS-DA),variable importance in the projection (VIP),and non-parametric test to determine intergroup differential metabolites.Abnormal metabolic pathways were determined by Metaboanalyst.Results Compared with the health control group,contents of fourteen substances like inositol,uric acid,stearic acid,and so on decreased,and mino-malonic acid content increased in asthma episode children (P ＜0.05).The process was mainly involved in 5 metabolic pathways such as lysine degradation and biosynthesis,pyruvate metabolism,and so on.Compared with the non-PHOFS group in bronchial asthma episode,contents of nine substances like oxalic acid,L-threonine,pyrimidine,and so on decreased in the PHOFS group (P ＜0.05).The process was mainly involved in 5 metabolic pathways such as pentose phosphate pathway,inositol phosphate metabolism,and so on.Conclusions Urinary metabolites are different in infantile bronchial asthma episode and healthy children.Metabolic biomarkers and pathways exist in different syndromes in bronchial asthma episode.关键词
代谢组学/小儿支气管哮喘/痰热阻肺/气相色谱—质谱联用/证候学标记物Key words
metabolomics/infantile bronchial asthma/phlegm-heat obstructing Fei/gas chromatography-mass spectrometry/syndrome marker引用本文复制引用
陶嘉磊,汪受传,田曼,梁慧,谢彤,林丽丽,戴启刚..小儿支气管哮喘发作期证候学标记物的代谢组学研究[J].中国中西医结合杂志,2017,37(3):319-325,7.基金项目
江苏省儿童呼吸疾病(中医药)重点实验室开放课题(No.JKLPRD201401) （中医药）
