中国中西医结合杂志2017，Vol.37Issue(3)：345-350,6.DOI:10.7661/CJIM.2017.03.0345
人参水提液对TAMs与肿瘤细胞共培养体系肺癌A549细胞生物学行为的影响
Effect of Water Extract of Ginseng on Biological Bechaviors of Lung Cancer A549 Cells and the Expression of F-actin in Co-culture System of TAMs and A549 Cells
摘要
Abstract
Objective To study the effect of Water Extract of Ginseng (WEG) on the proliferation/metastasis of lung cancer A549 cells and the expression of F-actin in co-culture system of tumor associated macrophages (TAMs) and A549 cells.Methods Human acute leukemia mononuclear strain THP-1 was induced to become TAMs using Phorbol-12-myristate-13-acetate (PMA) combined IL-4 and IL-13.The supernant of TAMs and A549 cells were co-cultured.A co-culture model was set up by simulating microenvironment of lung cancer.Then cells were divided into the blank control group (A549),the coculture group (A549 +TAMs),high,middle,and low dose WEG groups (TAMs +A549 + high,middle,and low dose WEG).The effects of WEG on the proliferation/metastasis of lung cancer A549 cells and the expression of F-actin under various conditions were detected using MTT method,Real time cell analysis (RTCA),and high content screening (HCS).Results Compared with the blank control group,the proliferation of A549 cells was obviously increased,cell migration was obviously elevated,and the area of cell skeleton was markedly enlarged in the TAMs + A549 group,with statistical difference (P ＜ 0.05).Compared with the TAMs +A549 group,the proliferation and migration of A549 cells were inhibited,the area of cell skeleton and the number of microfilaments were reduced dose-dependently (P ＜0.05).Conclusion WEG could effectively inhibit the proliferation and migration of A549 cells,which might be achieved by adjusting immunoactivities of TAMs,and further it affected biological behaviors of tumor cells.关键词
人参水提液/肿瘤相关巨噬细胞/肺癌A549细胞/共培养体系Key words
Water Extract of Ginseng/tumor associated macrophages/lung cancer A549 cell/co-culture system引用本文复制引用
高静,毕蕾,江玉翠,杨烨,李变英,陈卫平..人参水提液对TAMs与肿瘤细胞共培养体系肺癌A549细胞生物学行为的影响[J].中国中西医结合杂志,2017,37(3):345-350,6.基金项目
国家自然科学基金资助项目(No.81503486,No.81273638) （No.81503486,No.81273638）
江苏省高校自然科学研究项目(No.14KJD360004) （No.14KJD360004）
江苏省高校优势学科建设工程项目(中西医结合) （中西医结合）
