北华大学学报（自然科学版）2017，Vol.18Issue(4)：454-458,5.DOI:10.11713/j.issn.1009-4822.2017.04.007
五味子多糖与泽泻提取物联合应用对小鼠急性酒精性肝损伤的保护作用
Protective Effects of Schisandra Polysaccharides and Extract of Alisma Orientals on Acute Liver Injury Induced by Alcohol in Mice
摘要
Abstract
Objective To observe the protective effects of Schisandra polysaccharides and Alisma orientalis extract on the acute alcohol-induced liver injury in mice.Method 50 Mice were randomly divided into 5 groups,control group,alcoholic liver injury model group,Schisandra and Alisma (2:1) treatment group,Schis-andra and Alisma (1:1) treatment group,and Schisandra and Alisma (1:2) treatment group.The mice in Schisandra and Alisma treated groups were intragastrically administered with Schisandra polysaccharides and Alisma orientalis extract (100 mg/kg),and those in the control and model group were given the same volume solvent for 30 days in the same way.1 Hour after the last administration,50% ethanol (12 mL/kg) was administered to the mice in model group and Schisandra and Alisma treated groups.16 Hours later,blood and liver samples of mice were collected for the calculation of liver indexes of mice in each group and the detection of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in serum as well as the contents of reduced glutathione (GSH) and triglyceride (TG) in liver tissues.Pathological changes of the mice liver were observed by hematoxylin-eosin staining (HE).Results Compared with the alcohol-induced liver injury model,Schisandra and Alisma extract could significantly reduce the liver index in mice (P<0.05) and the levels of ALT and AST in serum (P<0.05),significantly elevate the content of GSH (P<0.01),significantly reduce the content of TG in liver tissues (P<0.05),and significantly improve the pathological changes of the liver tissues.Conclusion Application of Schisandra polysaccharides and Alisma orientalis extract should have a protective effect on the acute alcohol-induced liver injury in mice.关键词
五味子/泽泻/急性酒精性肝损伤Key words
Schisandra/Alisma orientalis/acute alcohol-induced liver injury分类
医药卫生引用本文复制引用
苑荣爽,孙卉,杨雪晗,孙靖辉,李贺,陶雪,陈建光,王春梅..五味子多糖与泽泻提取物联合应用对小鼠急性酒精性肝损伤的保护作用[J].北华大学学报（自然科学版）,2017,18(4):454-458,5.基金项目
吉林省科技厅医药产业推进计划项目(20140311052YY) （20140311052YY）
吉林省科技厅产业技术创新战略联盟项目(20150309004YY). （20150309004YY）
