北华大学学报（自然科学版）2017，Vol.18Issue(4)：466-469,4.DOI:10.11713/j.issn.1009-4822.2017.04.010
风湿性心脏瓣膜钙化的二尖瓣组织中巨噬细胞和炎性因子表达分析
Distribution of Macrophage and Expression of Inflammatory Cytokines in Mitral Valve Tissues with Rheumatic Valve Calcification
摘要
Abstract
Objective To investigate the distribution of macrophages and the expression of inflammatory cytokines in mitral valve tissues with rheumatic valve calcification.Method A total of 13 patients with rheumatic heart disease undergoing mitral valve replacement were selected as the study group and a total of 5 patients with end-stage heart disease were enrolled in the study.Mitral valve tissue specimens were collected,all macrophages were labeled with CD68,M1 and M2 macrophages were labeled with iNOS and CD163,and the infiltration of macrophages in calcified mitral valve tissues was observed.The expression of macrophage-associated inflammatory mediators (eNOS,IL-10,Arg-1,macrophage colony stimulating factor) was observed in calcified mitral valve.Results CD68 expression score in the study group was higher than that in the control group and the difference was statistically significant (P<0.05),and there were a lot of endometrial interstitial macrophage infiltration layer;the expression score of iNOS-positive M1 macrophages was higher than that in the control group and the difference was statistically significant (P<0.01);the expression score of CD163-positive M2 macrophages in the study group was lower than that in the control group,with a statistically significant difference (P<0.05).The expression score of eNOS in the study group was higher than that in the control group,showing a statistically significant difference (P<0.01).The expression scores of IL-10 and Arg-1 in the study group were lower than those in the control group,and the difference was statistically significant (P<0.01).The expression score of macrophage colony stimulating factor in the study group was significantly lower than that in the control group (P<0.01).Conclusion Rheumatic mitral valve calcification is characterized by the infiltration of M1 macrophages,in which the inflammation can be directly promoted through the up-regulation of eNOS and other inflammatory factors,and indirectly by inhibiting IL-10 and other inflammatory factors.The down-regulation of macrophage colony-stimulating factor can reduce the transformation from M1 type to M2 type and maintain the inflammatory response for a long time.关键词
风湿性心脏病/二尖瓣/瓣膜钙化/巨噬细胞Key words
rheumatic heart disease/mitral valve/valve calcification/macrophage分类
医药卫生引用本文复制引用
马微..风湿性心脏瓣膜钙化的二尖瓣组织中巨噬细胞和炎性因子表达分析[J].北华大学学报（自然科学版）,2017,18(4):466-469,4.基金项目
上海市卫生局面上项目(20144055). （20144055）
