北京中医药大学学报2017，Vol.40Issue(6)：456-462,7.DOI:10.3969/j.issn.1006-2157.2017.06.003
脾虚1号方对脾虚证功能性消化不良大鼠小肠葡萄糖转运蛋白1的影响
The eEffects of Pixu Yihao Fang on small intestinal GLUT1 of small intestine in spleen-deficient rats with functional dyspepsia rats with spleen deficiency
摘要
Abstract
Objective To investigate the effects of Pixu Yihao Fang (PXYHF, No.1 Formula for Spleen Deficiency) on the expression of Glucose Transporter (GLUT1)protein (GLUT1) and mRNA in small intestine tissue of FD spleen-deficient rats and functional dyspepsia (FD) the intervention of Pixu I Fang.Methods 70 10-day-old male SD rat pups were randomly divided into normal control group (n=10), FD model group (single model, n=10), and spleen-deficient FD model group (double model, n=50).The normal control group received intragastric administration of 0.2 mL of 2% sucrose, while the single and double model groups were given 0.2 mL of 0.1 % iodoacetamide (IA)IA in 2 % sucrose, for 6 days.6 weeks after birth consecutive days, the Modified multiple platform method (MMPM) was also applied to establish double model for 14 days after week 6.These spleen-deficient FD rats were randomly divided into double model control group, domperidone group, PXYHF low-dose, medium-dose and high-dose dose groups, and were orally gavaged with 10 mL/(kg·d) of distilled water, 3.125 mg/(kg·d) of domperidone, 1.275, 2.55, 5.1 g/(kg·d) of PXYHF respectively for 14 days.Immunohistochemistry, Western-blot and RT-PCR methods were used to measure the GLUT1 protein expression in liver tissues.Results Compared with the normal group, average optical density of GLUT1 protein in small intestinal mucosal tissue, the expression of GLUT1 protein and GLUT1 mRNA in small intestinal tissue of double model group were decreased (P<0.05, P<0.01);Compared with double model group, average optical density of GLUT1 protein in small intestinal mucosa tissue, expression of GLUT1 protein and GLUT1 mRNA in small intestinal tissue of low-dose PXYHF were increased (P<0.01).Conclusion The expression of GLUT1 gene and protein decreased in the FD rats with spleen deficiency, and Pixu Yihao Fang could increase the expression of GLUT1 gene and protein.关键词
脾虚1号方/脾虚证/功能性消化不良/葡萄糖转运蛋白1/大鼠Key words
Pixu Yihao Fang/pattern of spleen deficiency/functional dyspepsia/glucose transporter Ⅰ/rats分类
医药卫生引用本文复制引用
吕林,王凤云,唐旭东,马祥雪,尹晓岚,石啸双..脾虚1号方对脾虚证功能性消化不良大鼠小肠葡萄糖转运蛋白1的影响[J].北京中医药大学学报,2017,40(6):456-462,7.基金项目
国家重点基础研究发展计划(973计划)项目(No.2013CB531703),国家自然科学基金项目(No.81503567),中国博士后科学基金项目(No.2015M1227,2016T90195)Key Project of Chinece National Programs for Foundamental Research and Development(973 Program)(No.2013CB531703), National Natural Science Foundation of China(No.81503567), China Postdoctoral Science Foundation(No.2015M1227,2016T90195) （973计划）
