北京中医药大学学报2017，Vol.40Issue(6)：483-491,9.DOI:10.3969/j.issn.1006-2157.2017.06.007
脑络欣通对大鼠脑缺血再灌注后海马mRNA的特异性表达及Wnt/β-catenin的影响
Effects of Naoluo Xintong on mRNA, wnt/β-catenin in hippocampus of MCAO/R rats
摘要
Abstract
Objective To investigate the mechanism of protective effects of Naoluo Xintong (NLXT) on cerebral ischemia-reperfusion injury in rats.Methods 144 male SD rats were randomly divided into 4 groups (n =36 in each group): sham-operation group, model group, normal control group and treatment group.Each group was randomly further divided into 1/3/7d subgroups (n=12 in each subgroup).Treatment group was administered NLXT after modeling on the 1st, 3rd, and 7th days, and normal control group was given Tongxinluo on the 1st, 3rd, and 7th days.The treatment group and control group was given 10 mL/(kg·d) of NLXT liquid (concentration =0.26 g/mL) and Tongxinluo liquid (concentration =0.02 g/mL) respectively.The model group and sham-operation group were orally gavaged with isopyknic physiological saline solution on the 1st, 3rd, 7th days.Regional cerebral blood flow (rCBF) of cerebral infarction side was measured through laser Doppler Flowmetry (LDF).All the rats were dissected and hippocampal tissues were removed from their brains.Then DG area of rat's hippocampus was measured by using HE staining.MiRNA9a, miRNA15a, and miRNA124 in the hippocampus were detected with RT-PCR;Wnt3a, Wnt5a and β-catenin was detected by using Western blot method.Results Compared with the sham-operation group, rats of the model group presented apparent cerebral ischemia;levels of miRNA124, Wnt3a, Wnt5a, and β-catenin expression in hippocampal area significantly increased (P<0.01;Levels of miRNA9a and miRNA15 expression markedly decreased.Compared with the model group, the blood flow in brain increased significantly on the 1st, 3rd, and 7th days (P<0.01;Levels of miRNA9a, miRNA15a, Wnt3a, Wnt5a, and β-catenin expression in hippocampal area increased significantly;miRNA124 expression decreased significantly.Conclusion NLXT had a good effect of treating cerebral injury of MCAO/R rats.This medicine could increase the cerebral blood flow, miRNA9a, miRNA15a, Wnt3a, Wnt5a, and β-catenin expressions, and decrease miRNA124 expression in rat hippocampus, i.e.inhibiting ischemia-reperfusion cerebral impairment in rats.关键词
脑络欣通/益气活血通络法/脑缺血再灌注/mRNA/Wnt/β-catenin/大鼠Key words
Naoluo Xintong granules/supplementing qi and nourishing blood to regulate meridians method/ischemia reperfusion injury/mRNA/wnt/β-catenin/rats分类
医药卫生引用本文复制引用
邓勇,王键,谭辉,何玲..脑络欣通对大鼠脑缺血再灌注后海马mRNA的特异性表达及Wnt/β-catenin的影响[J].北京中医药大学学报,2017,40(6):483-491,9.基金项目
国家中医药管理局"新安王氏内科流派传承工作室"建设项目(No.国中医药人教函{2012}228号),国家自然科学基金青年项目(No.81603523),安徽省高校自然科学研究重点项目(No.KJ2016A404)Construction Project of "Xin'an Wang Medical School Inheritance Studio" by the State Administration of Traditional Chinese Medicine(No.{2012}228), Youth Project of National Natural Science Foundation of China(No.81603523), Key University Science Research Project of Anhui Province (No.KJ2016A404) （No.国中医药人教函{2012}228号）
