北京中医药大学学报2017，Vol.40Issue(6)：492-497,6.DOI:10.3969/j.issn.1006-2157.2017.06.008
老年期痴呆同病异证3个典型证候Tau蛋白磷酸化位点的特异性研究
Study on the specificity of Tau protein phosphorylation sites in three typical patterns of senile dementia based on the same disease with different patterns
摘要
Abstract
Objective To observe Tau protein phosphorylation in rats with three typical patterns of senile dementia, analyzing and summarizing the specificity of phosphorylation.Methods We ligated and dissected the left and right carotid arteries at one week's interval with intraperitoneal injection of D-galactose to establish senile dementia model in rats.Those rats then received the intervention of excessive sexual and physical exertion, high-fat feeding milk and low temperature environment respectively to establish the rat models of senile dementia with three different patterns (group K: kidney deficiency and essence loss pattern;group P: retention of phlegm and turbid substance in orifices pattern;group C: congealing cold and blood stasis pattern).Blood lipid, blood rheology and serum testosterone were measured to identify whether those model was built successfully.Different positions of Tau phosphorylation sites were measured with Western blot method.Results In the Tau protein proline-rich region, compared with the diseased groups, phosphorylation level increased significantly at the sites of 205 and 231 in group P.This increase was also present at sites of 181, 205, and 231 in group K and 205, 231 and 262 in group C.At the C-terminal region, compared with the diseased group, phosphorylation level of P group and K group were significantly increased at the sites of 404, and the increase of group C was the highest among those groups.Conclusion There is specificity at the Tau protein phosphorylation sites in each typical senile dementia pattern.This study may reveal the scientific rationale for the presence of different patterns in the same disease.关键词
老年期痴呆/证候/病证结合/Tau蛋白磷酸化位点/大鼠Key words
senile dementia/syndrome/combination of disease and pattern/Tau protein phosphorylation sites/rats分类
医药卫生引用本文复制引用
郭斌,郭蕾,韩诚,秦亚莉,师卫斌,侯江琪..老年期痴呆同病异证3个典型证候Tau蛋白磷酸化位点的特异性研究[J].北京中医药大学学报,2017,40(6):492-497,6.基金项目
国家自然科学基金项目(No.81173165)National Natural Science Foundation of China(No.81173165) （No.81173165）
