草业科学2017，Vol.34Issue(6)：1204-1214,11.DOI:10.11829/j.issn.1001-0629.2016-0396
CRISPR/Cas9基因组编辑技术及其在草类植物中的应用
CRISPR/Cas9 system and its prospective application in grass
摘要
Abstract
The CRISPR/Cas9 system is a new, precise genome editing technology, which has received wide attention from the scientific community globally.CRISPR/Cas9 is widely utilised for studying gene functions, because it enables precise modification of specific gene sites, including knockout, insertion, and substitution.CRISPR/Cas9 has many advantages, including convenience, precision, efficiency, and a wide selection of target sites.Presently, this technology is applied in all the field of life sciences, including microorganisms, plants, animals, and human gene therapy.The present article briefly introduces the course of development of CRISPR/Cas9 technology, mechanism of action, technical advantage, functions, and its applications.It summarizes the progresses in targetted plant genome editing, and discusses the potential applications of this process in research on grass species, including studies on functional genomics, gene metabolism and regulation, and genetic improvement, which provides a useful reference for the later researches and application of this field.关键词
CRISPR/Cas9/基因组编辑/草类植物/定点敲除/基因功能/遗传改良Key words
CRISPR/Cas9 system/genome editing/grass/targeted mutagenesis/gene function research/genetic improvement分类
农业科技引用本文复制引用
王丹,周美亮,李金博,孙占敏,吴燕民..CRISPR/Cas9基因组编辑技术及其在草类植物中的应用[J].草业科学,2017,34(6):1204-1214,11.基金项目
国家自然科学基金项目(31372361) （31372361）
国家重点基础研究发展"973"项目(2014CB138701) （2014CB138701）
广东省省级科技计划项目(2015B090904008) （2015B090904008）
