重庆医学2017，Vol.46Issue(16)：2178-2182,5.DOI:10.3969/j.issn.1671-8348.2017.16.006
黄桷树叶总黄酮提取物对A549细胞的保护作用研究
Protective effects of total flavonoids extraction from ficus lacor leaves on A549 cells
摘要
Abstract
Objective To investigate the extraction method of total flavonoids from the leaves of ficus lacor and the protec tive effects of extraction on the cellular damage to provide a basis for the research on the phamaceutical value of ficus lacor leaves.Methods The ethanol extraction method was adopted to extract the total flavonoids in the leaves of ficus lacor and the extraction efficiency was calculated with rutin as the standard.The rotenone induced human lung adenocarcinoma cellular damage served as the model,then the influencesof the extraction on the cellular viability,cellular morphology,production of reactive oxygen species (ROS) and apoptosis were researched.Results The extraction efficiency of total flavonoids in the leaves of ficus lacor by 60％ ethanol was 5.02％;the extraction at the concentration of 32 mg/L could significantly inhibit the decrease of cell viability,cellular shape change,ROS production and apoptosis of A549 cells induced by 100μg/L rotenone.Conclusion The ethanol extraction method can be used to extract the total flavonoids in the leaves of ficus lacor and the extraction has the protective effects on the A549 cellular dam age induced by rotenone,the leaves of ficus lacor have the potential for further researching its pharmaceutical value.关键词
黄桷树叶/总黄酮提取物/A549人肺腺癌细胞/活性氧/细胞凋亡Key words
leaf of Ficus Lacor/total flavonoids extraction/A549 human lung adenocarcinoma cell/reactive oxygen species/cell apoptosis分类
医药卫生引用本文复制引用
汪洋,胡魁,陈玲,苏燕,马佳卉,肖虹,白群华..黄桷树叶总黄酮提取物对A549细胞的保护作用研究[J].重庆医学,2017,46(16):2178-2182,5.基金项目
重庆市科委前沿与应用基础研究计划项目基金(cstc2014jcyjA10019) （cstc2014jcyjA10019）
重庆医科大学大学生科学研究与创新项目基金(201306) （201306）
重庆市创新创业计划项目(201310631007). （201310631007）
