重庆医学2017，Vol.46Issue(16)：2215-2217,3.DOI:10.3969/j.issn.1671-8348.2017.16.017
原发性肝癌患者肿瘤转移与T细胞亚群及其细胞因子的相关性分析
Relationship between tumor metastasis with T cell subsets and cytokines in patients with primary hepatocellular carcinoma
胡丛岗 1汤国军 1王建军 1童骎1
作者信息
- 1. 金华广福医院胃肠外科,浙江金华321000
- 折叠
摘要
Abstract
Objective To investigate the correlation between tumor metastasis with T cell subsets and cytokines in the pa tients with primary hepatocellular carcinoma (HCC).Methods Ninety-seven cases of primary HCC were prospectively collected from January 2014 to January 2016 and assigned into the metastasis group (38 cases) and non-metastasis group(59 cases) according to whether suffering from metastasis.Surgical specimens were obtained from all pauents and flow cytometry was used to detect the CD4+,CD8+ T cell proportion in HCC tissue,paracancerous tissues and peripheral blood.Moreover,ELISA was adopted to detect the peripheral blood IL-6,IL-10,TNF-α and IFN-γ levels.Results Compared with the non-metastasis group,the CD4+ T cell proportion of HCC tissue in the metastasis group was significantly increased(P=0.02),while the CD8+ T cells were significantly decreased (P=0.015).There was no statistical difference in CD4+ T cells proportion in the paracancerous tissue between the two groups (P=0.328).However the CD8+ T cells proportion of paracancerous tissue in the metastasis group was significantly higher than that in the non-metastasis group (P=0.021).There was no statistically significant difference in the proportion of CD8+ T cells in peripheral blood of the two groups (P=0.362).The proportion of CD4+ T cells in peripheral blood of the metastatic group was significantly lower than that in non-metastasis group (P=0.032).When compared with non-metastasis group,the metastasis group got a decreased level of peripheral blood IL-6 (P =0.012);while the IL-10 level was significantly increased (P =0.006);the TNF-α level and IFN-γ level were significantly decreased(P=0.000,P=0.035).Conclusion The patients with primary HCC have obvious T cell subsets and cytokines imbalance.关键词
原发性肝癌/肿瘤转移/T细胞亚群/细胞因子/免疫抑制Key words
primary liver cancer/tumor metastasis/T cell subsets/cytokines/immune suppression分类
医药卫生引用本文复制引用
胡丛岗,汤国军,王建军,童骎..原发性肝癌患者肿瘤转移与T细胞亚群及其细胞因子的相关性分析[J].重庆医学,2017,46(16):2215-2217,3.
