重庆医学2017，Vol.46Issue(17)：2313-2315,3.DOI:10.3969/j.issn.1671-8348.2017.17.003
缺血/再灌注大鼠心肌PPAR-γ表达变化对再灌注心律失常的影响
Effects of myocardial PPAR-gamma expression change on reperfusion arrhythmias in ischemia/reperfusion rats
摘要
Abstract
Objective To investigate the effects of PPAR-gamma expression on reperfusion arrhythmias in different precon ditioning myocardial ischemia/reperfusion(I/R) animal model.Methods Thirty-two SD rats were randomly divided into 4 groups(n =8):rosiglitazone+ I/R group (ROS group),GW9662 + I/R group(GW group),I/R group and sham group (Sham group).The I/ R animal model was constructed by ligation of the left anterior descending coronary artery,with ischemia for 30 min and reperfusion for 120 min.The dynamic limb Ⅱ lead electrocardiogram monitoring was performed;PPAR-gamma mRNA was detected by fluorescent quantitative PCR and the change of PPAR-gamma protein expression was detected by Western blot.Results The increasing range of QRS wave width detected before operation,at 30 min of ischemia,at 1,2 h of reperfusion from large to small were in turn the ROS group,I/R group,GW group and Sham group;the reperfusion arrhythmia score in the ROS group was significantly higher than that in the other groups(P＜0.05),while the GW group was relatively reduced.The expression level of PPAR-gamma mRNA in the ROS group detected by fluorescent quantitative PCR was significantly up-regulated(P＜0.05),while which in the GW group was down-regulated compared with the I/R group and ROS group(P＜0.05).The expression of PPAR-gamma protein was similar to that of PPAR-gamma mRNA.Conclusion Up-regulation of myocardial PPAR-gamma expression may increase the occurrence of reperfusion arrhythmias in myocardial I/R animal model.关键词
心肌缺血/心肌再灌注损伤/PPAR-γ/再灌注心律失常/罗格列酮Key words
myocardial ischemia/myocardial reperfusion injury/PPAR-γ/reperfusion arrhythmias/rosiglitazone分类
医药卫生引用本文复制引用
陈华,莫新玲,何学恕,谢婷..缺血/再灌注大鼠心肌PPAR-γ表达变化对再灌注心律失常的影响[J].重庆医学,2017,46(17):2313-2315,3.基金项目
广西壮族自治区卫生厅自筹课题(Z2013492). （Z2013492）
