重庆医学2017，Vol.46Issue(17)：2326-2329,4.DOI:10.3969/j.issn.1671-8348.2017.17.007
电针调控新西兰兔骨关节炎性疼痛及对脊髓IL-17、IL-17R表达的影响
Electroacupuncture regulates pain and its effect on IL-17 and IL-17R expression of spinal cord in New Zealand rabbit with osteoarthritis
摘要
Abstract
Objective To investigate whether electroacupuncture(EA) regulates the pain in New Zealand rabbits with osteo arthritis by inhibiting the IL-17 and IL-17R expression in spinal cord.Methods New Zealand rabbits were randomly divided into the sham operation group,inflammation model group,sham operation+ IL-17 group,inflammation+ IL-17 group,inflammation + EA group and inflammation +sham EA group,8 cases in each group.The rabbit osteoarthritis model was established by using the papaya protease,IL-17 and IL-17 antiserum was injected into the spinal canal,The EA group selected the EA at Zusanli point(30 min,2 Hz continuous wave,1～ 2 mA),the sham EA group adopted the non-acupoint stimulation.Then the pain threshold of rabbits was detected.The mRNA expression of IL-17 and IL-17R in rabbit spinal cord tissue was detected by RT-qPCR and the protein expression was detected by Western-Bolt.Results The rabbit pain threshold was significantly decreased after constructing the New Zealand osteoarthritis model(P＜0.05),and the spinal canal injection of IL-17 could significantly decrease the pain threshold of sham operation New Zealand rabbit(P＜0.05),while the spinal canal injection of IL-17 antiserum could significantly increase the pain threshold of osteoarthritis model rabbit.Selecting EA at Zusanli point could significantly enhance the rabbit pain threshold (P＜0.05).The mRNA and protein expression of IL-17 and IL-17R of the osteoarthritis model group were significantly increased (P＜0.05),while the expressions were significantly decreased after EA stimulation(P＜0.05).Conclusion The pain occurrence of New Zealand rabbit osteoarthritis may involve the IL-17 abnormal expression in spinal cord.EA may regulate the osteoarthritis pain by inhibiting the expression of IL-17 and IL-17R in spinal cord.关键词
电针/新西兰兔/骨炎性疼痛/白细胞介素-17/白细胞介素-17RKey words
electroacupuncture/New Zealand rabbit/osteoarthritis pain/IL-17/1L-17R分类
医药卫生引用本文复制引用
程红,杨敏..电针调控新西兰兔骨关节炎性疼痛及对脊髓IL-17、IL-17R表达的影响[J].重庆医学,2017,46(17):2326-2329,4.基金项目
四川省中医药管理局青年中医药课题基金资助(2014K055). （2014K055）
