重庆医学2017，Vol.46Issue(18)：2453-2455,3.DOI:10.3969/j.issn.1671-8348.2017.18.002
MNES对脑损伤后昏迷大鼠PFC去甲肾上腺素α1受体表达的影响
Effect of median nerve electrical stimulation on expression of α1-adrenergic receptor in prefrontal cortex of coma rats induced by traumatic brain injury
摘要
Abstract
Objective To investigate the wake-promoting action of median nerve electrical stimulation(MNES) in coma rats induced by traumatic brain injury(TBI) and its influence on the expression of α1-adrenergic receptor(α1 R) in the prefrontal contex (PFC).Methods Seventy-two healthy Sprague Dawley(SD) rats were randomly divided into the control group,sham-stimulated group(TBI),stimulated group (TBI+ MNES) and antagonist group(TBI+ OX1R antagonist +MNES).The control group had no any treatment.The TBI coma rat models were prepared in the other 3 groups.The sham stimulated group had no treatment.The antagonist group was injected with orexin receptor-l(OX1R) antagonist SB334867 into lateral ventricle,and both the antagonist group and stimulated group received MNES treatment.Then the behavior changes of rats in each group were observed and the α1 R expression level in PFC was detected by using the immunohistochemistry technique.Results Thirteen rats in the stimulated group and 8 rats in the antagonist group revived,while only 4 rats in the TBI group.The α1R levels from low to high were the blank control group,sham-stimulated group,antagonist group and stimulated group,showing the increasing trend,and the difference was statistically significant(P＜0.05).Conclusion MNES can improve the rat consciousness level after TBI coma,and its mechanism may be related with up-regulating the α1 R expression level in PFC area,moreover Orexin-A participates in this regulation process.关键词
正中神经/电刺激疗法/脑损伤/昏迷/促醒/受体,肾上腺素能α1/食欲素AKey words
median nerve/electric stimulation therapy/brain injuries/coma/wake-promoting/receptors, adrenergic, alpha-1/Orexin-A分类
医药卫生引用本文复制引用
陈琴,杜青,冯珍..MNES对脑损伤后昏迷大鼠PFC去甲肾上腺素α1受体表达的影响[J].重庆医学,2017,46(18):2453-2455,3.基金项目
国家自然科学基金资助项目(81260295) （81260295）
江西省自然科学基金资助项目(20132BAB205063). （20132BAB205063）
