重庆医学2017，Vol.46Issue(18)：2456-2458,3.DOI:10.3969/j.issn.1671-8348.2017.18.003
星形胶质细胞活化后瞬时受体电位通道6在TBI中的作用
Effect of TRPC6 after astrocytes activation in traumatic brain injury
摘要
Abstract
Objective To explore the role of astrocytes transient receptor potential channel 6(TRPC6) in rat brain injury (TBI).Methods Thirty-nine male Sprague-Dawley(SD) rats were divided into the sham operation group,injury group and deferoxamine(DFX) group(n=13).According to the previous model construction scheme established by our research group Feeney method,the rat brain impact injury model was established.The Morris water maze test was performed and the defected brain volume was measured.The immunofluorescence was adopted to detect the co-expression of TRPC6 and GFAP.Then Western blot was performed.Results The defected brain volume after TBI in the DFX group was significantly decreased compared with the injury group [(115.35 ± 13.70)mm3 vs.(209.99 ± 16.70)mm3]] (P＜0.05).The Morris water maze test found that the platform search strategy and search time in the DFX group were(3.13 ± 0.35) and(36.15 ± 26.63)s,which were significantly improve d compared with (2.13±0.64) and(110±47.34)s in the injury group(P＜0.05).The immunofluorescence found that GFAP in the DFX group was highly expressed,moreover the co-expression with TRPC6 was increased.Western blot found that TRPC6 in the DFX group was significantly down-regulated(P＜0.01).Conclusion In rat TBI early stage,strocytes are activated after DFX treatment and TRPC6 is highly expressed,playing a neuroprotective role.关键词
颅脑损伤/神经胶质原纤维酸性蛋白质/瞬时受体电位通道/去铁胺Key words
traumatic brain injury/glial fibrillary acidic protein/transient receptor potential channels/deferoxamine分类
医药卫生引用本文复制引用
张礼均,冯华..星形胶质细胞活化后瞬时受体电位通道6在TBI中的作用[J].重庆医学,2017,46(18):2456-2458,3.基金项目
国家自然科学基金青年基金项目(30901545) （30901545）
重庆市自然科学基金项目(cstc2015jcyjA1051) （cstc2015jcyjA1051）
重庆市卫计委临床重点专科项目(渝卫医函2015-517). （渝卫医函2015-517）
