渔业研究2017，Vol.39Issue(3)：165-171,7.DOI:10.14012/j.cnki.fjsc.2017.03.001
抗原钥孔戚血蓝蛋白(KLH)对金鱼(Carassius auratus)体液免疫的影响研究
Effect of keyhole limpet hemocyanin(KLH)as antigen on humoral immunity in goldfish(Carassius auratus)
摘要
Abstract
In order to provide enough reference material for enacting immune strategy of goldfish(Carassius auratus),KLH was used as antigen to immunize it with adjuvant Montanide ISA 763 A,and the serum antibody level was detected to evaluate the humoral immunoresponse.The experiment was carried out under 15~18℃ and 150 healthy fish with body weight of(20±3)g were randomly divided into 3 groups.Group Ⅰ was immunized with(KLH+Montanide ISA 763A),Group Ⅱ was merely immunized with KLH and Group Ⅲ was intraperitoneal injected with equivalent PBS as control.Antiserum was sampled after using KLH plus ISA adjuvant or not from the immunized fish three times,and the antibody variation was evaluated by ELISA.As a result,the antibody level in Group Ⅰ and Group Ⅱ were high.The antibody level of Group Ⅰ was still high after the first and second booster immunization,while that of Group Ⅱ were decreased and approached to the level of Group Ⅲ.We found the fish immunized with KLH plus ISA could acquire a long and stable immunity,while the fish merely immunized with KLH could get a short and transitory one.This study also showed the goldfish possessed well humoral immunoresponse in hypothermia situation.关键词
钥孔戚血蓝蛋白/金鱼/体液免疫/MontanideISA763AKey words
keyhole limpet hemocyanin；goldfish；humoral immunity；Montanide ISA 763 A分类
农业科技引用本文复制引用
郭睿,杨小强,江小斌,王洪城,陈秀霞,方冬兰,龚晖..抗原钥孔戚血蓝蛋白(KLH)对金鱼(Carassius auratus)体液免疫的影响研究[J].渔业研究,2017,39(3):165-171,7.基金项目
2016年福州市渔业病虫害研究、检测项目(2200209) （2200209）
2016年福州市水产养殖新品种引进推广项目(2130106). （2130106）
