分析测试学报2017，Vol.36Issue(5)：645-649,5.DOI:10.3969/j.issn.1004-4957.2017.05.011
普鲁士蓝@石墨烯-壳聚糖纳米复合物用于构建microRNA电化学传感器的研究
Development of microRNA Electrochemical Biosensor Based on Prussian Blue@Graphene-Chitosan Nanocomposite Prepared by in situ Deposition
摘要
Abstract
A microRNA(miR,miRNA) electrochemical biosensor was proposed in the present research.Firstly,graphene(GN) was dispersed in the chitosan(Chi) solution to promote the conductivity of chitosan film.Then,prussian blue (PB) was in situ deposited on GN to obtain the PB@GN-Chi nanocomposite.The obtained nanocomposites were modified on the surface of electrode.Compared with the PB@GN-Chi prepared by ultrasonic method,the prepared composite could effectively prevent PB leaking into detection solution and display a prominent stability when modified on the electrode.The capture probe was subsequently modified by amino group and covalently linked to the modified electrode via glutaraldehyde.After blocked by hexanethiol,the miRNA biosensor was successfully prepared.The prepared PB@GN-Chi nanocomposite was observed with transmission electron microscope,and the electrochemical characteristics of the miRNA biosensor were investigated by cyclic voltammetry and differential pulse voltammetry.Under the optimal conditions,the biosensor displayed a linear relationship between current response and the logarithm of concentration of miR-21 in the range of 2.8-2.8 × 104 pmol/L.The detection limit was calculated to be 0.87 pmoL/L.The prepared biosensor owns good stability and repeatability,and could be employed to detect miR-21 in human serum sample.关键词
电化学生物传感器/普鲁士蓝/石墨烯/纳米复合物/microRNAKey words
electrochemical biosensor/prussian blue/graphene/nanocomposite/microRNA分类
化学化工引用本文复制引用
苏会岚,李德长,张瑞林,梅浩,汤科..普鲁士蓝@石墨烯-壳聚糖纳米复合物用于构建microRNA电化学传感器的研究[J].分析测试学报,2017,36(5):645-649,5.基金项目
国家自然科学基金资助项目(81401757) （81401757）
发育与再生四川省重点实验室项目(SYS16-004) （SYS16-004）
成都医学院科研创新团队(CYTD16-03) （CYTD16-03）
