分析测试学报2017，Vol.36Issue(5)：693-696,4.DOI:10.3969/j.issn.1004-4957.2017.05.021
核磁共振氢谱法测定甘露聚糖肽中多糖的含量
Determination of Polysaccharide Content in Mannatide by 1H-NMR
吴梦琪 1张文清 1徐志珍 1宛燕飞 2张力 2夏玮1
作者信息
- 1. 华东理工大学上海市功能性材料化学重点实验室,上海200237
- 2. 成都利尔药业有限公司,四川成都610083
- 折叠
摘要
Abstract
A proton-nuclear magnetic resonance(1H-NMR) method was established for the determination of polysaccharide content in mannatide using potassium acid phthalate as the internal standard.Effects of some experiment parameters were investigated.The optimal conditions were selected as follows:relaxation delay time(d1):1 s;transient number(ns):16.The results indicated that,under the optimal conditions,the good precision and repeatability for the method were obtained.The polysaccharide content in mannatide,determined by the 1 H-NMR method with inner standard model,was similar to that of the ultraviolet spectrophotometry method.The established method was simple,specific and repeatable.Therefore,it could be used not only for the determination of polysaccharide content in mannatide,but also for detection of other kinds of polysaccharide with definite structures.关键词
核磁共振氢谱/多糖含量/甘露聚糖肽Key words
1H nuclear magnetic resonance/polysaccharide content/mannatide分类
数理科学引用本文复制引用
吴梦琪,张文清,徐志珍,宛燕飞,张力,夏玮..核磁共振氢谱法测定甘露聚糖肽中多糖的含量[J].分析测试学报,2017,36(5):693-696,4.
