福州大学学报（自然科学版）2017，Vol.45Issue(3)：446-453,8.DOI:10.7631/issn.1000-2243.2017.03.0446
交替假单胞菌BYS-2产褐藻胶裂解酶条件研究
Optimization of alginate lyase-producing conditions by Pseudoalteromonas sp. BYS-2
摘要
Abstract
With sodium alginate as the only carbon source of primary screening medium, one of the mi-croorganisms that producing alginate lyase had been isolated from abalone aquaculture water, which was identified as Pseudoalteromonas sp. BYS-2 via morphological characteristics and 16S rDNA sequence analysis. Based on the results of fermentation experiments, the optimal liquid medium consisted of those compounds as follow:sodium alginate 1 g·L-1, glucose 0.5 g·L-1, yeast extract 10 g·L-1, soybean cake powder 3 g·L-1, (NH4)2HPO43 g·L-1, pH 8.0. The optimal fermentation conditions included:inoculation 2%(volume fraction) , medium volume 50 mL /250 mL, incubate temperature 20℃ and shaking speed 200 r·min-1 . Fermenting about 24 h under these conditions, alginate lyase activity could reach 5.29 U·mL-1 which was 2.37 times higher than before optimization (1.57 U·mL-1).关键词
褐藻胶裂解酶/筛选/交替假单胞菌属/产酶条件优化Key words
alginate lyase/screening/Pseudoalteromonas/enzyme production optimization分类
生物科学引用本文复制引用
徐凡,林娟,叶秀云,朱凡,许鑫琦..交替假单胞菌BYS-2产褐藻胶裂解酶条件研究[J].福州大学学报（自然科学版）,2017,45(3):446-453,8.基金项目
国家海洋局海洋公益性行业科研专项基金(201305015),福建省海洋高新产业发展专项基金(闽海洋高新[2013]20号) （201305015）
