广东药学院学报2017，Vol.33Issue(2)：167-171,5.DOI:10.16809/j.cnki.2096-3653.2017011301
Box-Behnken响应面法优化乌榄叶中酚类成分的提取工艺研究
Optimization on extraction process of phenolic constituents from Canarium pimela L.leaves by Box-Behnken design
摘要
Abstract
Objective To optimize the extraction process of phenolic constituents from Canarium pimela L. leaves. Methods The single-factor experiments coupled with Box-Behnken design were applied to evaluate the yields of index components and extraction ratio. Ethanol concentration,solid-liquid ratio,extraction method and time were selected as independent parameters. Results The optimal extraction conditions were as follows: ethanol concentration of 56%,liquid to solid ratio of 50∶1 (mL∶g) and refluxing for 78 min. The experimental comprehensive score was 98.60±0.47 which was close to the predicted value of 97.58. Conclusion The Box-Behnken design methodology was reasonable and practical with a good consistency between the mathematical model and the experimental data,which was suitable for optimizing the extraction process of the C. pimela L. leaves.关键词
乌榄叶/黄酮/酚类/Box-Behnken响应面法/提取工艺Key words
Canarium pimela L./flavonoids/phenolic/Box-Behnken design/extraction分类
医药卫生引用本文复制引用
方小爱,谢清春,陈燕忠,何伟,班俊峰,梁燕玲,董艳芬,吕竹芬..Box-Behnken响应面法优化乌榄叶中酚类成分的提取工艺研究[J].广东药学院学报,2017,33(2):167-171,5.基金项目
广东省科技计划项目(2013B020311020) （2013B020311020）
2015广东省普通高校创新团队建设项目(自然科学)(2015KCXTD026) （自然科学）
