广东药学院学报2017，Vol.33Issue(2)：221-225,5.DOI:10.16809/j.cnki.2096-3653.2017020901
铁皮石斛复方制剂的抗氧化和延缓衰老作用研究
Antioxidant and anti-aging effect of Dendrobium officinale formula in Caenorhabditis elegans
摘要
Abstract
Objective To study the antioxidant and anti-aging effect of Dendrobium officinale formula in Caenorhabditis elegans models.Methods The antioxidant activity of D.officinale formula was first determined in vitro using DPPH and hydroxyl free radical scavenging assays.Then food clearance assay was performed to determine the appropriate dosages of D.officinale formula to be used in C.elegans.Survival rate and lifespan of C.elegans were then determined under oxidative stress induced by paraquat and under normal growth conditions,respectively.The antioxidant enzyme activities,reactive oxygen species (ROS) levels and malondialdehyde (MDA) content were measured in C.elegans exposed to paraquat.Results D.officinale formula was capable of scavenging both DPPH and hydroxyl free radicals in vitro.The formula increased the survival rate of C.elegans under oxidative stress induced by 50 mmol/L paraquat and extend the lifespan under normal growth conditions.When C.elegans was exposed to 2 mmol/L paraquat,D.officinale formula was able to reduce ROS level and increase superoxide dismutase (SOD) activity,but the catalase activity and MDA content were not changed.Conclusion D.officinale formula was able to increase the survival rate under oxidative stress and extend the lifespan under normal conditions in C.elegans,possibly through increasing SOD activity.关键词
铁皮石斛/秀丽线虫/抗衰老/抗氧化/SODKey words
D.officinale/C.elegans/anti-aging/ROS levels/antioxidant/SOD分类
医药卫生引用本文复制引用
高大可,亓兵,汪靓怡,肖小春,翟旭峰,余雪松,黄泽波..铁皮石斛复方制剂的抗氧化和延缓衰老作用研究[J].广东药学院学报,2017,33(2):221-225,5.基金项目
广东省普通高校国际暨港澳台合作创新平台及国际合作重大项目(2015KGJHZ022) （2015KGJHZ022）
中央财政支持地方高校发展专项资金(粤财政[2016]202号) （粤财政[2016]202号）
