广东药学院学报2017，Vol.33Issue(2)：241-244,4.DOI:10.16809/j.cnki.2096-3653.2016122001
鬼臼苦素抗Ph+急性淋巴细胞白血病细胞Sup-B15的作用及其机制
Effects of picropodophyllin on Ph+ acute lymphoblastic leukemia Sup-B15 cells and its underlying mechanisms
摘要
Abstract
Objective To investigate the effects of picropodophyllin (PPP) on philadelphia chromosome positive (Ph+) acute lymphoblastic leukemia (Ph+ ALL) Sup-B15 cells and its underlying mechanisms.Methods MTS assay was used to analyze the effect of PPP and imatinib mesylate (IM) on the proliferation of Sup-B15 cells.The cell cycle distribution after PPP and IM treatment of Sup-B15 cells were determined by flow cytometry.The effect of PPP on the expression of proliferation and cell cycle-associated proteins,and the phosphorylation and expression of IGF1R was determind by Western blot.Results PPP and IM inhibited the proliferation of Sup-B15 cells in a dose-and time-dependent manner,and the inhibitory effect of PPP was stronger than IM at the same doses.Cell cycle analysis showed that PPP treatment for 12 and 24 h resulted in significant accumulation at the G2/M phase and decreased at S phase of Sup-B15 cells.PPP treatment increased p53 and cyclin B1 protein expression,and reduced p21 and c-myc protein expression,but had no obvious effect on phosphorylation and total protein levels of IGF1R.Conclusion PPP can effectively inhibit Sup-B15 cell proliferation and induce G2/M cell cycle arrest,which is associated with upregulation of p53 and cyclin B1 expression and inhibition of p21 and c-myc expression.关键词
鬼臼苦素/Ph+急性淋巴细胞白血病/Sup-B15Key words
picropodophyllin/Ph+ acute lymphoblastic leukemia/Sup-B15分类
医药卫生引用本文复制引用
陈永恒,廖芬芳,程琳,侯宇,王伟章..鬼臼苦素抗Ph+急性淋巴细胞白血病细胞Sup-B15的作用及其机制[J].广东药学院学报,2017,33(2):241-244,4.基金项目
国家自然青年科学基金项目(81100369) （81100369）
广东省自然科学基金项目(2014A030313580) （2014A030313580）
广东省科技计划项目(2013B021800084) （2013B021800084）
大学生创新创业训练计划项目(1057313028) （1057313028）
