广东药学院学报2017，Vol.33Issue(2)：250-254,5.DOI:10.16809/j.cnki.2096-3653.2017022601
冬凌草甲素对LPS诱导Raw264.7巨噬细胞炎症反应的抑制作用
Inhibition of oridonin on LPS-induced inflammation response in Raw264.7 macrophages
摘要
Abstract
Objective To investigate the effect of oridonin on lipopolysaccharide (LPS)-induced inflammation response in Raw264.7 macrophage cell line.Methods The optimal concentration of oridonin on Raw264.7 macrophages was determined by CCK8 method.The inflammation models were established by LPS stimulation and divided into four groups including the normal control,the LPS group,the experimental group (oridonin pretreatment + LPS) and the positive control (dexamethasone pretreatment+LPS).The changes of TNF-α,IL-1β,IL-6,IL-10 and TLR4 mRNA levels were measured by real-time quantitative PCR.The expression of NF-κB p65 and phosphorylated p65 (p-p65) were detected by western blot.Results At the optimal concentration (10 μmol/L),oridonin pretreatment significantly inhibited the expression of TNF-α,IL-1β and IL-6 mRNA induced by LPS and increased the expression of IL-10 mRNA in Raw264.7 cells.Oridonin also inhibited the expression of TLR4 and activation of NF-κB translocation into nucleus.Conclusion Oridonin can inhibit the expression of proinflammatory cytokines induced by LPS and exert anti-inflammation immune mechanism through TLR4-NF-κB signaling pathway.关键词
冬凌草甲素/Raw264.7巨噬细胞/Toll样受体4/NF-κBKey words
oridonin/macrophages/Toll-like receptor 4/nuclear factor-κB分类
医药卫生引用本文复制引用
郐一贺,顾取良,邓慧君,亓翠玲,王丽京..冬凌草甲素对LPS诱导Raw264.7巨噬细胞炎症反应的抑制作用[J].广东药学院学报,2017,33(2):250-254,5.基金项目
国家自然科学基金项目(31471290) （31471290）
广东省科技计划项目(2013B021800082) （2013B021800082）
