国际妇产科学杂志2017，Vol.44Issue(3)：300-303,4.
微小RNA-3619-5p通过靶向作用于p21基因对卵巢癌细胞生长的影响
Effect of MicroRNA-3619-5p on the Growth of Ovarian Cancer Cells by Targeting p21 Gene
任志敏 1古雅丽 1陈慧萍 1钱进1
作者信息
- 1. 450012 河南大学附属郑州市妇幼保健院病理科
- 折叠
摘要
Abstract
Objective: To investigate the effect of microRNA-3619-5p (miR-3619-5p) overexpression on the up-regulation of p21 gene in ovarian cancer cell lines A2780 and SKOV3 and its effect on ovarian cancer cell growth. Methods:miR-3619-5p mimics (experimental group) or dsControl (control group) were transiently transfected into ovarian cancer cell lines A2780 and SKOV3 using Lipofectamine 3000 respectively. The expression of p21, CDK4 and Cyclin D1 mRNA was detected by real-time quantitative polymerase chain reaction (qRT-PCR). The expression of p21, CDK4 and Cyclin D1 was detected by Western blot. Flow cytometry was used to detect the cell cycle distribution and cell apoptosis in the control and experimental groups. EdU proliferation assay and colony formation assay were conducted to analyze the proliferation ability of transfected cells. Results:Compared with dsControl group, the expression of p21 mRNA, CDK4 mRNA and Cyclin D1 mRNA in cell lines transfected with miR-3619-5p were significantly increased (P<0.01). Western blot results were consistent with qRT-PCR results. Compared with the dsControl group, the percentage of cells in S phase and G 2/M phase decreased significantly after transfection of miR-3619-5p, the proportion of cells in G0/G1 phase was significantly increased, and the apoptosis rate was significantly increased. The proliferation ability of ovarian cancer cells transfected with miR-3619-5p was significantly lower than that of the dsControl group, as demonstrated by the EdU proliferation assay and colony-forming assay (P<0.05). Conclusions:miR-3619-5p can significantly inhibit the growth of ovarian cancer cells by activating the expression of p21 protein in ovarian cancer cells.关键词
微RNAs/卵巢肿瘤/p21蛋白/细胞增殖/细胞凋亡Key words
MicroRNAs/Ovarian neoplasms/p21 protein/Cell proliferation/Apoptosis引用本文复制引用
任志敏,古雅丽,陈慧萍,钱进..微小RNA-3619-5p通过靶向作用于p21基因对卵巢癌细胞生长的影响[J].国际妇产科学杂志,2017,44(3):300-303,4.
