广西医学2017，Vol.39Issue(6)：795-798,4.DOI:10.11675/j.issn.0253-4304.2017.06.11
拓扑异构酶ⅡαmRNA在乳腺癌中的表达及其临床意义与基因富集分析
Expression of topoisomerase Ⅱ alpha mRNA in breast cancer,its clinical significance and gene set enrichment analysis
摘要
Abstract
Objective To explore the expression of topoisomerase Ⅱ alpha(TOP2A) mRNA in breast cancer and its clinical significance,and to predict the gene sets regulated by TOP2A influencing the development of breast cancer.Methods GSM42568 expression data and corresponding clinical information were collected from Gene Expression Omnibus database.The expression of TOP2A mRNA was assayed in 104 cases of breast cancer tissues and 17 cases of normal breast tissues.The correlation between TOP2A mRNA and clinicalopathological features of breast cancer was analyzed.The survival was compared between TOP2A mRNA high-expression group and TOP2A mRNA low-expression group.Gene Set Enrichment Analysis was used to predict the relevant gene sets regulated by TOP2A in the samples with TOP2A mRNA high-expression.Results The expression level of TOP2A mRNA in breast cancer tissues was significantly higher than that in the normal breast tissues(P<0.05).In the patients with breast cancer,the expression of TOP2A mRNA correlated with T stage(P<0.05),and did not correlate with age,tumor grade,lymph node metastasis or estrogen receptor status(P>0.05).The median survival time and disease-free survival rate in the TOP2A mRNA high-expression group were lower than those in the TOP2A mRNA low-expression group(P<0.05).Relevant gene sets including base excision repair,cell cycle,DNA replication,proteasome,RNA degradation and splice were enriched in the samples of TOP2A mRNA high-expression.Conclusion TOP2A may be a carcinogen of breast cancer.It can affect the prognosis of patients with breast cancer,and may become a new target for breast cancer.关键词
乳腺癌/拓扑异构酶Ⅱα/预后/基因富集分析/基因集Key words
Breast cancer/Topoisomerase Ⅱ alpha/Prognosis/Gene Set Enrichment Analysis/Gene sets分类
医药卫生引用本文复制引用
杨迪,曾健,李富,罗铭,梁滨琦,袁慧明..拓扑异构酶ⅡαmRNA在乳腺癌中的表达及其临床意义与基因富集分析[J].广西医学,2017,39(6):795-798,4.基金项目
广西医疗卫生适宜技术研究与开发课题(S201415-03) （S201415-03）
