海南医学院学报2017，Vol.23Issue(10)：1402-1405,4.DOI:10.13210/j.cnki.jhmu.20170503.012
膀胱癌组织中MCP-1、VEGF、CUGBP1表达相关性及其与癌细胞生长的关系
MCP-1, VEGF and CUGBP1 expression correlation in bladder cancer tissue and their relationship with the cancer cell growth
摘要
Abstract
Objective: To study the MCP-1, VEGF and CUGBP1 expression correlation in bladder cancer tissue and their relationship with the cancer cell growth.Methods: A total of 80 patients with primary bladder cancer who accepted radical operation for bladder cancer in our hospital between July 2012 and July 2016 were selected, the bladder cancer tissue and tissue adjacent to carcinoma were collected, western-blot method was used to determine the MCP-1, VEGF and CUGBP1 protein expression and fluorescent quantitative PCR method was used to determine proliferation and apoptosis gene mRNA expression.Pearson test was used to assess the correlation among MCP-1, VEGF and CUGBP1 as well as the correlation of MCP-1, VEGF and CUGBP1 protein expression with proliferation and apoptosis gene mRNA expression.Results: MCP-1, VEGF and CUGBP1 protein expression in bladder cancer tissue were significantly higher than those in tissue adjacent to carcinoma, and the protein expression of the three were positively correlated (P<0.05).Proliferation genes KPNA2, MRPS5 and YAP mRNA expression in bladder cancer tissue were higher than those in tissue adjacent to carcinoma while NDPK-A and Sox1 mRNA expression were lower than those in tissue adjacent to carcinoma, and apoptosis genes Bcl-2, Rce1 and cIAP-1 mRNA expression were higher than those in tissue adjacent to carcinoma while Bax, STAG2 and RUNX3 mRNA expression were lower than those in tissue adjacent to carcinoma (P<0.05).MCP-1, VEGF and CUGBP1 protein expression in bladder cancer tissue were positively correlated with proliferation genes KPNA2, MRPS5 and YAP mRNA expression while negatively correlated with NDPK-A and Sox1 mRNA expression, and were positively correlated with apoptosis genes Bcl-2, Rce1 and cIAP-1 mRNA expression while negatively correlated with Bax, STAG2 and RUNX3 mRNA expression (P<0.05).Conclusions: MCP-1, VEGF and CUGBP1 expression in bladder cancer tissue promote each other, act on tumor cell proliferation and apoptosis genes together, accelerate the proliferation and inhibit the apoptosis of tumor cells.关键词
膀胱癌/单核细胞趋化蛋白-1/血管内皮生长因子/CUG连接蛋白1/增殖/凋亡Key words
Bladder cancer/Monocyte chemoattractant protein-1/Vascular endothelial growth factor/CUG-binding protein 1/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
杨昌俊,陈早庆,刘文姣..膀胱癌组织中MCP-1、VEGF、CUGBP1表达相关性及其与癌细胞生长的关系[J].海南医学院学报,2017,23(10):1402-1405,4.基金项目
东莞市科技局项目(201410515000573) （201410515000573）
It is supported by Project of Dongguan City Science and Technology Office (201410515000573). （201410515000573）
