环境工程学报2017，Vol.11Issue(6)：3813-3822,10.DOI:10.12030/j.cjee.201603082
芘和苯并[a]芘复合污染土壤中的环糊精-微生物连续修复
Continuous remediation of pyrene and benzo [a] pyrene-contaminated soil by cyclodextrin-microbial remediation
摘要
Abstract
Soil was artificially contaminated by spiking it with pyrene (Pyr) and benzo[a] pyrene (BaP) at concentrations of 200 mg· kg-1 and 100 mg · kg-1,respectively.Then,hydroxypropyl-β-cyclodextrin (HPCD)and Pyr-degrading bacteria were used to study the remediation effect on the Pyr and BaP and any changes in microbial community structure,in the soil.After adding 10％ (w/w) HPCD and 5％ (v/w) Pyr-degrading bacteria to the artificially contaminated soil,the degradation rates of both Pyr and BaP increased.The effect of HPCD and Pyr-degrading bacteria added simultaneously was stronger than adding either HPCD or Pyr-degrading bacteria alone.After 14 weeks,the activity of three enzymes (dehydrogenase,polyphenol oxidase,and fluorescein diacetate esterase) in the soil was enhanced,except in the control treatment (CK),with the effects of the HPCD + Pyr-degrading bacteria treatment (MB) being the most obvious.This was similar to the trend in degradation.The Tenax 6 h extraction amount of Pyr and BaP was used to characterize their bioavailability,and the results showed that the Tenax 6 h extraction amounts of Pyr ranked as follows:HPCD treatment (M) ＞CK ＞ MB＞Pyr-degrading bacteria treatment (CKB).This means that HPCD significantly enhanced the bioavailability of Pyr,as the bioavailability of Pyr from CKB and MB was inhibited,most obviously in CKB.The Tenax 6 h extraction amount of BaP ranked as follows:M＞MB ＞ CK＞CKB,meaning not only did HPCD increase BaP's bioavailability,but MB did as well,and the bioavailability of BaP from CKB was also inhibited.High-throughput sequencing showed that after 10 weeks of culture,the samples after M,MB,CK,and CKB had similar soil bacterial compositions.Pyr-degrading bacteria increased the bacterial diversity and HPCD reduced it;therefore both changed the community structure of the soil bacteria.关键词
芘/苯并[a]芘/羟丙基-β-环糊精/降解菌/TenaxKey words
pyrene/benzo[a] pyrene/hydroxypropyl-β-cyclodextrin/degrading bacteria/Tenax分类
资源环境引用本文复制引用
王立红,丁克强,刘廷凤,杨凤,郭光..芘和苯并[a]芘复合污染土壤中的环糊精-微生物连续修复[J].环境工程学报,2017,11(6):3813-3822,10.基金项目
国家自然科学基金资助项目(41271329) （41271329）
南京工程学院大学生科技创新基金2016年挑战杯支撑项目(TZ20161204) （TZ20161204）
南京工程学院人才引进项目(YKJ201328) （YKJ201328）
